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Physiological and biochemical studies on pollen embryogenesis of Nicotiana species
http://hdl.handle.net/2241/6850
http://hdl.handle.net/2241/685083f9edf2-9bf8-454e-92d8-c5771d472400
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B0409.pdf (194.4 kB)
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1.pdf (2.1 MB)
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| Item type | Thesis or Dissertation(1) | |||||
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| 公開日 | 2007-07-25 | |||||
| タイトル | ||||||
| タイトル | Physiological and biochemical studies on pollen embryogenesis of Nicotiana species | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
京, 正晴
× 京, 正晴 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A method for the induction of a high rate of cell division and embryogenesis of Nicotiana rustica pollen was developed. Binucleate pollen grains were fractionated by Percoll density gradient (35/45%) centrifugation and cultured in 0.4 M mannitol at 30ﾟC (the 1st culture). After 3 days in culutre pollen was recollected by a 2nd Percoll fractionation (0/30%) and transferred to and cultured in a medium containing the Murashige-Skoog (MS) macro-elements, 0.4 M mannitol, 40 mM galactose, 3 mM glutamine, and 5μM ABA for 10 days (the 2nd culutre). The cell population consisting of about 80% dividing pollen was transferred to a MS medium containing 0.4 M mannnitol, 3mM glutamine, and no phytohormone (the 3rd culuture), where about 40% of dividing pollen developed into embryos or embryogenic calli. | |||||
| 言語 | en | |||||
| 書誌情報 |
発行日 1987 |
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| 取得学位 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士（理学） | |||||
| 取得学位 | ||||||
| 言語 | en | |||||
| 学位名 | Doctor of Philosophy in Science | |||||
| 学位授与大学 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 12102 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 筑波大学 | |||||
| 言語 | en | |||||
| 学位授与機関名 | University of Tsukuba | |||||
| 学位授与年度 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 1987 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 1987-10-31 | |||||
| 報告番号 | ||||||
| 学位授与番号 | 乙第409号 | |||||
