@phdthesis{oai:tsukuba.repo.nii.ac.jp:00008377,
 author = {京, 正晴 and Kyo, Masaharu},
 month = {},
 note = {A method for the induction of a high rate of cell division and embryogenesis of Nicotiana rustica pollen was developed. Binucleate pollen grains were fractionated by Percoll density gradient (35/45%) centrifugation and cultured in 0.4 M mannitol at 30ﾟC (the 1st culture). After 3 days in culutre pollen was recollected by a 2nd Percoll fractionation (0/30%) and transferred to and cultured in a medium containing the Murashige-Skoog (MS) macro-elements, 0.4 M mannitol, 40 mM galactose, 3 mM glutamine, and 5μM ABA for 10 days (the 2nd culutre). The cell population consisting of about 80% dividing pollen was transferred to a MS medium containing 0.4 M mannnitol, 3mM glutamine, and no phytohormone (the 3rd culuture), where about 40% of dividing pollen developed into embryos or embryogenic calli., 1987},
 school = {筑波大学, University of Tsukuba},
 title = {Physiological and biochemical studies on pollen embryogenesis of Nicotiana species},
 year = {1987}
}