{"created":"2021-03-01T06:43:46.281023+00:00","id":8377,"links":{},"metadata":{"_buckets":{"deposit":"97381d3a-e357-483a-9c1c-1aff35f6ce92"},"_deposit":{"id":"8377","owners":[],"pid":{"revision_id":0,"type":"depid","value":"8377"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00008377","sets":["3:233:254"]},"author_link":["34831"],"item_12_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1987","bibliographicIssueDateType":"Issued"}}]},"item_12_date_granted_46":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"1987-10-31"}]},"item_12_degree_grantor_44":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"筑波大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"University of Tsukuba"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"12102","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_12_degree_name_43":{"attribute_name":"取得学位","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"},{"subitem_degreename":"Doctor of Philosophy in Science"}]},"item_12_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A method for the induction of a high rate of cell division and embryogenesis of Nicotiana rustica pollen was developed. Binucleate pollen grains were fractionated by Percoll density gradient (35/45%) centrifugation and cultured in 0.4 M mannitol at 30ﾟC (the 1st culture). After 3 days in culutre pollen was recollected by a 2nd Percoll fractionation (0/30%) and transferred to and cultured in a medium containing the Murashige-Skoog (MS) macro-elements, 0.4 M mannitol, 40 mM galactose, 3 mM glutamine, and 5μM ABA for 10 days (the 2nd culutre). The cell population consisting of about 80% dividing pollen was transferred to a MS medium containing 0.4 M mannnitol, 3mM glutamine, and no phytohormone (the 3rd culuture), where about 40% of dividing pollen developed into embryos or embryogenic calli.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_12_description_45":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"1987","subitem_description_type":"Other"}]},"item_12_dissertation_number_47":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙第409号"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"京, 正晴","creatorNameLang":"ja"},{"creatorName":"Kyo, Masaharu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34831","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2013-12-18"}],"displaytype":"detail","filename":"B0409.pdf","filesize":[{"value":"194.4 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tsukuba.repo.nii.ac.jp/record/8377/files/B0409.pdf"},"version_id":"f27763b3-632b-4636-b572-bd26d19b2155"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2013-12-18"}],"displaytype":"detail","filename":"1.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tsukuba.repo.nii.ac.jp/record/8377/files/1.pdf"},"version_id":"2e1c28f8-f350-4ab3-a357-f0c7b7c2b334"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Physiological and biochemical studies on pollen embryogenesis of Nicotiana species","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Physiological and biochemical studies on pollen embryogenesis of Nicotiana species","subitem_title_language":"en"}]},"item_type_id":"12","owner":"1","path":["254"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-07-25"},"publish_date":"2007-07-25","publish_status":"0","recid":"8377","relation_version_is_last":true,"title":["Physiological and biochemical studies on pollen embryogenesis of Nicotiana species"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-01T07:50:52.585012+00:00"}