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A decay of gap junctions between neuronal progenitor cells and ganglion cell differentiation during retinal regeneration of adult newt (Cynops pyrrhogaster)
http://hdl.handle.net/2241/6826
http://hdl.handle.net/2241/68260d2edb7e-6a9a-40cf-8d99-2a0d99ad3f76
| Item type | Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-07-25 | |||||
| タイトル | ||||||
| タイトル | A decay of gap junctions between neuronal progenitor cells and ganglion cell differentiation during retinal regeneration of adult newt (Cynops pyrrhogaster) | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
大井, 華子
× 大井, 華子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Adult newt(Cynops phyrrhogaster) possesses the ability to regenerate a new functional retina following complete removal of the original retina even in adult life. Regenerating retinas were divided into five stages('early', 'intermediate-I,-II and -III', and 'late' stages) on their basis of the morphological appearance. In the present study, I prepared living slice preparations of the adult newt retina at different stages of regenation, and investigated the functional differentiation of retinal neurons, especially ganglion cells, during regeneratio using whole-cell patch-clamp methods. Cells from which currents were recorded were identified by Lucifer Yellow and/or biocytin fills. In the 'early' regenerating newt retina, it has been reported that mitotically active progenitor cells were electrically inexcitable and strongly coupled each other through gap junction. In Chapter 1, I raised a question whether loss of the gap junctional coupling correlates with ganglion cell differentiation. For this purpose, I measured changes in the gap-junction currents and appearance and maturation of voltage-gated Na+ currents during retinal regeneration. All progenitor cells examined in the 'intermediate-I and-II' regenerating retinas, like those in the 'early' regenerating retina(Chiba & Saito, 2000), were characterized by oval or slender shape and not exhibit voltage-gated Na+ currents. They showed gap-junction currents under conditions that suppressed nonjunctional currents flowing through the plasma membrane. The presence of gap junctions between progenitor cells was confirmed by tracer coupling following intracellular injection of biocytin. Voltage-gated Na+ currents were first detected in premature ganglion cells with rounded somata located ar the most proximal level of the 'intermediate-II' rgenerating retina. On average, the maximum Na+ current amplitude was 432 pA and the activation threshold of the current was about -45 mV. These premature ganglion cells did not exhibit ･･･ | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Includes bibliographical references | |||||
| 言語 | en | |||||
| 書誌情報 |
発行日 2004 |
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| 取得学位 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士（理学） | |||||
| 取得学位 | ||||||
| 言語 | en | |||||
| 学位名 | Doctor of Philosophy in Science | |||||
| 学位授与大学 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 12102 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 筑波大学 | |||||
| 言語 | en | |||||
| 学位授与機関名 | University of Tsukuba | |||||
| 学位授与年度 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2003 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2004-02-29 | |||||
| 報告番号 | ||||||
| 学位授与番号 | 甲第3294号 | |||||
