{"created":"2021-03-01T06:43:44.729768+00:00","id":8355,"links":{},"metadata":{"_buckets":{"deposit":"1ef154e9-4dd6-4c74-baac-0732caf4fb15"},"_deposit":{"id":"8355","owners":[],"pid":{"revision_id":0,"type":"depid","value":"8355"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00008355","sets":["3:233:254"]},"author_link":["34772"],"item_12_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004","bibliographicIssueDateType":"Issued"}}]},"item_12_date_granted_46":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2004-02-29"}]},"item_12_degree_grantor_44":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"筑波大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"University of Tsukuba"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"12102","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_12_degree_name_43":{"attribute_name":"取得学位","attribute_value_mlt":[{"subitem_degreename":"博士（理学）","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Science","subitem_degreename_language":"en"}]},"item_12_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Adult newt(Cynops phyrrhogaster) possesses the ability to regenerate a new functional retina following complete removal of the original retina even in adult life. Regenerating retinas were divided into five stages('early', 'intermediate-I,-II and -III', and 'late' stages) on their basis of the morphological appearance. In the present study, I prepared living slice preparations of the adult newt retina at different stages of regenation, and investigated the functional differentiation of retinal neurons, especially ganglion cells, during regeneratio using whole-cell patch-clamp methods. Cells from which currents were recorded were identified by Lucifer Yellow and/or biocytin fills. In the 'early' regenerating newt retina, it has been reported that mitotically active progenitor cells were electrically inexcitable and strongly coupled each other through gap junction. In Chapter 1, I raised a question whether loss of the gap junctional coupling correlates with ganglion cell differentiation. For this purpose, I measured changes in the gap-junction currents and appearance and maturation of voltage-gated Na+ currents during retinal regeneration. All progenitor cells examined in the 'intermediate-I and-II' regenerating retinas, like those in the 'early' regenerating retina(Chiba & Saito, 2000), were characterized by oval or slender shape and not exhibit voltage-gated Na+ currents. They showed gap-junction currents under conditions that suppressed nonjunctional currents flowing through the plasma membrane. The presence of gap junctions between progenitor cells was confirmed by tracer coupling following intracellular injection of biocytin. Voltage-gated Na+ currents were first detected in premature ganglion cells with rounded somata located ar the most proximal level of the 'intermediate-II' rgenerating retina. On average, the maximum Na+ current amplitude was 432 pA and the activation threshold of the current was about -45 mV. These premature ganglion cells did not exhibit ･･･","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_12_description_45":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2003","subitem_description_type":"Other"}]},"item_12_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Includes bibliographical references","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_12_dissertation_number_47":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3294号"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大井, 華子","creatorNameLang":"ja"},{"creatorName":"Oi, 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