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Thermal aggregation of human immunoglobulin G in arginine solutions: Contrasting effects of stabilizers and destabilizers
http://hdl.handle.net/2241/00148650
http://hdl.handle.net/2241/0014865097a506a3-978c-416e-9730-7cdc2cf08e14
名前 / ファイル | ライセンス | アクション |
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IJBM_104 (751.5 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2017-11-09 | |||||
タイトル | ||||||
タイトル | Thermal aggregation of human immunoglobulin G in arginine solutions: Contrasting effects of stabilizers and destabilizers | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Yoshizawa, Shunsuke
× Yoshizawa, Shunsuke× Arakawa, Tsutomu× Shiraki, Kentaro |
|||||
著者別名 |
白木, 賢太郎
× 白木, 賢太郎 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Arginine is widely used as aggregation suppressor of proteins in biotechnology and pharmaceutics. However, why the effect of arginine depends on the types of proteins and stresses, including monoclonal antibodies, is still unclear. Here we investigated the precise processes of the thermal aggregation of human immunoglobulin G (IgG) in the presence of additives. As expected, arginine was the best additive to suppress the formation of insoluble aggregates during heat treatment, though it was unable to preserve the monomer content. A systematic analysis of the additives showed that sugars and kosmotropic ion inhibit the formation of soluble oligomers. These behaviors indicate that the thermal aggregation of IgG occurs by (i) the formation of soluble oligomers, which is triggered by the unfolding process that can be stabilized by typical osmolytes, and (ii) the formation of insoluble aggregates through weak cluster–cluster interactions, which can be suppressed by arginine. Understanding the detailed mechanism of arginine will provide useful information for the rational formulation design of antibodies. | |||||
書誌情報 |
International journal of biological macromolecules 巻 104, p. 650-655, 発行日 2017-11 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01418130 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1153092X | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 28647523 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ijbiomac.2017.06.085 | |||||
権利 | ||||||
権利情報 | © 2017. | |||||
権利 | ||||||
権利情報 | This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Elsevier |