{"created":"2021-03-01T07:23:23.217955+00:00","id":43764,"links":{},"metadata":{"_buckets":{"deposit":"165f8c07-0811-4dcb-9a04-aa6f93adcdd7"},"_deposit":{"id":"43764","owners":[],"pid":{"revision_id":0,"type":"depid","value":"43764"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00043764","sets":["117:306","3:62:5591:6154"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-11","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"655","bibliographicPageStart":"650","bibliographicVolumeNumber":"104","bibliographic_titles":[{"bibliographic_title":"International journal of biological macromolecules"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"白木, 賢太郎"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Arginine is widely used as aggregation suppressor of proteins in biotechnology and pharmaceutics. However, why the effect of arginine depends on the types of proteins and stresses, including monoclonal antibodies, is still unclear. Here we investigated the precise processes of the thermal aggregation of human immunoglobulin G (IgG) in the presence of additives. As expected, arginine was the best additive to suppress the formation of insoluble aggregates during heat treatment, though it was unable to preserve the monomer content. A systematic analysis of the additives showed that sugars and kosmotropic ion inhibit the formation of soluble oligomers. These behaviors indicate that the thermal aggregation of IgG occurs by (i) the formation of soluble oligomers, which is triggered by the unfolding process that can be stabilized by typical osmolytes, and (ii) the formation of insoluble aggregates through weak cluster–cluster interactions, which can be suppressed by arginine. Understanding the detailed mechanism of arginine will provide useful information for the rational formulation design of antibodies.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"28647523","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ijbiomac.2017.06.085","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017."},{"subitem_rights":"This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ "}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01418130","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1153092X","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yoshizawa, Shunsuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Arakawa, Tsutomu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shiraki, Kentaro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-12-01"}],"displaytype":"detail","filename":"IJBM_104.pdf","filesize":[{"value":"751.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"IJBM_104","url":"https://tsukuba.repo.nii.ac.jp/record/43764/files/IJBM_104.pdf"},"version_id":"c4a4d3c0-9539-4f28-9d95-ef147beac282"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Thermal aggregation of human immunoglobulin G in arginine solutions: Contrasting effects of stabilizers and destabilizers","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Thermal aggregation of human immunoglobulin G in arginine solutions: Contrasting effects of stabilizers and destabilizers"}]},"item_type_id":"5","owner":"1","path":["306","6154"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-11-09"},"publish_date":"2017-11-09","publish_status":"0","recid":"43764","relation_version_is_last":true,"title":["Thermal aggregation of human immunoglobulin G in arginine solutions: Contrasting effects of stabilizers and destabilizers"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:14:51.803505+00:00"}