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Studies on structures and expressions of highly repetitive sequences in the genomes of higher eukaryotes
http://hdl.handle.net/2241/6836
http://hdl.handle.net/2241/68364efef6a8-ede9-4d01-bc07-b0ae44994f18
名前 / ファイル | ライセンス | アクション |
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A0335.pdf (158.9 kB)
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DA00335.pdf
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Item type | Thesis or Dissertation(1) | |||||
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公開日 | 2007-07-25 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Studies on structures and expressions of highly repetitive sequences in the genomes of higher eukaryotes | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_db06 | |||||
タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
坂本, 和一
× 坂本, 和一 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Total DNAs isolated from several higher animals were transcribed in a HeLa cell extract. Transcription of the total DNAs from rat, mouse and hamster gives a discrete product of 6S RNA (about 180 nucleotides) in addtion to a smeared backgroud, whereas transcription from chick, calf and human DNAs gives a discrete 5S RNA (about 120 nucleotides). In view of the sensitivity to α-amanitin, these transcripts are produced by RNA polymerase III. Fingerprint analyses demonstrated that the 6S RNA species trancribed from rodent DNAs have conserved sequences. We compared the sequences of oligonucleotides of the 6S RNA produced from the mouse total DNA with those of a cloned mouse DNA fragment containing a representative type 2 Alu sequence (B2) which was characterized by Kominami et al. (1983). This result demonstrated that the 6S RNA transcribed from mouse total DNA was in fact the transcript of type 2 Alu sequences. The composition of the oligonucleotides of the 6S RNA transcribed from mouse total DNA seems to reflect precisely that of the consensus sequence of the type 2 Alu family proposed by Krayev et al. (1982). Fingerprints of in vitro transcripts of rat and hamster total DNAs showed nearly identical patterns to that of mouse DNA, confirming that rodents have the repetitive sequence with same length and simlar compositions of oligonucletides. Thus total DNA transcription provides a novel aproach to the detection and analysis of type 2 Alu sequences (Sakamoto et al. 1984). | |||||
言語 | en | |||||
書誌情報 |
発行日 1986 |
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取得学位 | ||||||
学位名 | 博士(理学) | |||||
取得学位 | ||||||
学位名 | Doctor of Philosophy in Science | |||||
学位授与大学 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 12102 | |||||
言語 | ja | |||||
学位授与機関名 | 筑波大学 | |||||
言語 | en | |||||
学位授与機関名 | University of Tsukuba | |||||
学位授与年度 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 1985 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 1986-03-25 | |||||
報告番号 | ||||||
学位授与番号 | 甲第335号 |