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Template Activating Factor-I α Regulates Retroviral Silencing during Reprogramming
http://hdl.handle.net/2241/00160175
http://hdl.handle.net/2241/00160175f5ebc4bf-25da-43ff-a119-f53de900c023
名前 / ファイル | ライセンス | アクション |
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Item type | Journal Article(1) | |||||
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公開日 | 2020-06-19 | |||||
タイトル | ||||||
タイトル | Template Activating Factor-I α Regulates Retroviral Silencing during Reprogramming | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
著者 |
西村, 健
× 西村, 健× 永田, 恭介× 福田, 綾× 久武, 幸司× Bui, Phuong Linh× Seminario Mondejar, Gonzalo× Kumar, Arun× Aizawa, Shiho× Murano, Kensaku× Hayashi, Yohei× Onuma, Yasuko× Ito, Yuzuru× Nakanishi, Mahito |
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抄録 | ||||||
内容記述 | Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) is accompanied by dramatic changes in epigenetic programs, including silencing of endogenous and exogenous retroviruses. Here, we utilized replication-defective and persistent Sendai virus (SeVdp)-based vectors to monitor retroviral silencing during reprogramming. We observed that retroviral silencing occurred at an early reprogramming stage without a requirement for KLF4 or the YY1-binding site in the retroviral genome. Insertional chromatin immunoprecipitation (iChIP) enabled us to isolate factors assembled on the silenced provirus, including components of inhibitor of histone acetyltransferase (INHAT), which includes the SET/TAF-I oncoprotein. Knockdown of SET/TAF-I in mouse embryonic fibroblasts (MEFs) diminished retroviral silencing during reprogramming, and overexpression of template activating factor-I α (TAF-Iα), a SET/TAF-I isoform predominant in embryonic stem cells (ESCs), reinforced retroviral silencing by an SeVdp-based vector that is otherwise defective in retroviral silencing. Our results indicate an important role for TAF-Iα in retroviral silencing during reprogramming. | |||||
書誌情報 |
en : Cell Reports 巻 29, 号 7, p. 1909-1922.e5, 発行日 2019-11 |
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ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 2211-1247 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
関連識別子 | https://doi.org/10.1016/j.celrep.2019.10.010 | |||||
PMID | ||||||
関連識別子 | 31722206 | |||||
権利情報 | ||||||
権利情報 | (c) 2019 The Author(s). | |||||
権利情報 | ||||||
権利情報 | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版者 | ||||||
出版者 | Cell Press |