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  1. 医学医療系 (Faculty of Medicine)
  2. 杉山 文博 (Sugiyama Fumihiro)
  1. 医学医療系 (Faculty of Medicine)
  2. 水野 聖哉 (Mizuno Seiya)
  1. 医学医療系 (Faculty of Medicine)
  2. 村田 知弥 (Murata Kazuya)
  1. 医学医療系 (Faculty of Medicine)
  2. DINH THI HUONG TRA (ディン ティ フォン チア)
  1. コンテンツタイプ (Contents Type)
  2. 雑誌発表論文等 (Journal article, etc.)
  3. E~
  4. Experimental animals

Generation of bicistronic reporter knockin mice for visualizing germ layers

http://hdl.handle.net/2241/00160022
f203c3b3-b8e6-444a-86a4-6ea334de8026
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EA_68-4-499.pdf EA_68-4-499 (2.6 MB)
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item type Journal Article(1)
公開日 2020-05-12
タイトル
言語 en
タイトル Generation of bicistronic reporter knockin mice for visualizing germ layers
言語
言語 eng
資源タイプ
タイプ journal article
著者 DINH, THI HUONG TRA

× DINH, THI HUONG TRA

WEKO 218873
筑波大学研究者総覧 0000004416

DINH, THI HUONG TRA

ja-Kana ディン, ティ フォン チア

en DINH, Thi Huong Tra

Search repository
村田, 知弥

× 村田, 知弥

WEKO 204930
e-Rad 60713485
筑波大学研究者総覧 0000004209

村田, 知弥

ja-Kana ムラタ, カズヤ

en MURATA, Kazuya

Search repository
水野, 聖哉

× 水野, 聖哉

WEKO 204885
e-Rad 10633141
筑波大学研究者総覧 0000003252

水野, 聖哉

ja-Kana ミズノ, セイヤ

en MIZUNO, Seiya

Search repository
杉山, 文博

× 杉山, 文博

WEKO 560
e-Rad 90226481
筑波大学研究者総覧 0000001632

杉山, 文博

ja-Kana スギヤマ, フミヒロ

en SUGIYAMA, Fumihiro

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Suzuki, Hayate

× Suzuki, Hayate

WEKO 218872

en Suzuki, Hayate

Search repository
Daitoku, Yoko

× Daitoku, Yoko

WEKO 218874

en Daitoku, Yoko

Search repository
Tanimoto, Yoko

× Tanimoto, Yoko

WEKO 218875

en Tanimoto, Yoko

Search repository
Kato, Kanako

× Kato, Kanako

WEKO 218876

en Kato, Kanako

Search repository
Azami, Takuya

× Azami, Takuya

WEKO 218877

en Azami, Takuya

Search repository
Ema, Masatsugu

× Ema, Masatsugu

WEKO 218878

en Ema, Masatsugu

Search repository
抄録
内容記述 Knockout mouse models are commonly used in developmental biology to investigate the functions of specific genes, and the knowledge obtained in such models has yielded insights into the molecular mechanisms underlying developmental processes. Gastrulation is the most dynamic process in embryogenesis during which differentiation into three germ layers occurs. However, the functions of genes involved in gastrulation are not completely understood. One major reason for this is the technical difficulty of embryo analysis to understand germ layer location. We have generated three reporter mouse strains in which the germ layers are distinguished by different fluorescent reporters. Using CRISPR/Cas9 genome editing in mouse zygotes, the fluorescent reporter genes, EGFP, tdTomato, and TagBFP including 2A peptide sequences were knocked into the appropriate sites before the stop codon of the Sox17 (endoderm marker), Otx2 (ectoderm marker), and T (mesoderm marker) genes, respectively. Founder mice were successfully generated in the Sox17-2A-EGFP, Otx2-2A-tdTomato, and T-2A-TagBFP knockin reporter strains. Further, homozygous knockin mice of all strains appeared morphologically normal and were fertile. On stereomicroscopic analysis, fluorescent signals were detected in a germ layer-specific manner from heterozygous embryos at embryonic day (E) 6.5–8.5 in all strains, and were immunohistochemically demonstrated to match their respective germ layer-specific marker protein at E7.5. Taken together, these observations suggest that the Sox17-2A-EGFP, Otx2-2A-tdTomato, and T-2A-TagBFP knockin reporter mice may be useful for comprehensive analysis of gene function in germ layer formation.
書誌情報 en : Experimental animals

巻 68, 号 4, p. 499-509, 発行日 2019-11
ISSN
収録物識別子 1341-1357
書誌レコードID
収録物識別子 AA11032321
PubMed番号
関連識別子
関連識別子 31189761
DOI
関連識別子
関連識別子 10.1538/expanim.19-0031
権利
権利情報 ©2019 Japanese Association for Laboratory Animal Science
権利
権利情報 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/4.0/>.
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値 publisher
出版者
出版者 Japanese Association for Laboratory Animal Science
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