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New assay method based on Raman spectroscopy for enzymes reacting with gaseous substrates
http://hdl.handle.net/2241/00157618
http://hdl.handle.net/2241/0015761837efb8b6-30a7-4cad-b584-8c02fea813fe
名前 / ファイル | ライセンス | アクション |
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PS_28-663 (717.8 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2019-08-29 | |||||
タイトル | ||||||
タイトル | New assay method based on Raman spectroscopy for enzymes reacting with gaseous substrates | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
重田, 育照
× 重田, 育照× Kawahara-Nakagawa, Yuka× Nishikawa, Koji× Nakashima, Satoru× Inoue, Shota× Ohta, Takehiro× Ogura, Takashi× Fukutani, Katsuyuki× Yagi, Tatsuhiko× Higuchi, Yoshiki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Enzyme activity is typically assayed by quantitatively measuring the initial and final concentrations of the substrates and/or products over a defined time period. For enzymatic reactions involving gaseous substrates, the substrate concentrations can be estimated either directly by gas chromatography or mass spectrometry, or indirectly by absorption spectroscopy, if the catalytic reactions involve electron transfer with electron mediators that exhibit redox‐dependent spectral changes. We have developed a new assay system for measuring the time course of enzymatic reactions involving gaseous substrates based on Raman spectroscopy. This system permits continuous monitoring of the gas composition in the reaction cuvette in a non‐invasive manner over a prolonged time period. We have applied this system to the kinetic study of the [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F. This enzyme physiologically catalyzes the reversible oxidation of H2 and also possesses the nonphysiological functions of H/D exchange and nuclear spin isomer conversion reactions. The proposed system has the additional advantage of enabling us to measure all of the hydrogenase‐mediated reactions simultaneously. Using the proposed system, we confirmed that H2 (the fully exchanged product) is concomitantly produced alongside HD by the H/D exchange reaction in the D2/H2O system. Based on a kinetic model, the ratio of the rate constants of the H/D exchange reaction (k) at the active site and product release rate (kout) was estimated to be 1.9 ± 0.2. The proposed assay method based on Raman spectroscopy can be applied to the investigation of other enzymes involving gaseous substrates. | |||||
書誌情報 |
Protein Science 巻 28, 号 3, p. 663-670, 発行日 2019-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09618368 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 30609080 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1002/pro.3569 | |||||
権利 | ||||||
権利情報 | © 2019 The Authors. Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society. | |||||
権利 | ||||||
権利情報 | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Published | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Wiley |