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Microtubule stabilizer reveals requirement of Ca2+-dependent conformational changes of microtubules for rapid coiling of haptonema in haptophyte algae
http://hdl.handle.net/2241/00155465
a912517a-bcbd-4084-b37b-2d63675e11ae
名前 / ファイル | ライセンス | Actions | |
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item type | Journal Article(1) | |||||||||||
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公開日 | 2019-05-17 | |||||||||||
タイトル | ||||||||||||
タイトル | Microtubule stabilizer reveals requirement of Ca2+-dependent conformational changes of microtubules for rapid coiling of haptonema in haptophyte algae | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
資源タイプ | ||||||||||||
タイプ | journal article | |||||||||||
著者 |
柴, 小菊
× 柴, 小菊× 中山, 剛× 石田, 健一郎
WEKO
104
× 稲葉, 一男× Nomura, Mami× Atsuji, Kohei× Hirose, Keiko× Yanase, Ryuji |
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抄録 | ||||||||||||
内容記述 | A haptonema is an elongated microtubule-based motile organelle uniquely present in haptophytes. The most notable and rapid movement of a haptonema is ‘coiling’, which occurs within a few milliseconds following mechanical stimulation in an unknown motor-independent mechanism. Here, we analyzed the coiling process in detail by high-speed filming and showed that haptonema coiling was initiated by left-handed twisting of the haptonema, followed by writhing to form a helix from the distal tip. On recovery from a mechanical stimulus, the helix slowly uncoiled from the proximal region. Electron microscopy showed that the seven microtubules in a haptonema were arranged mostly in parallel but that one of the microtubules often wound around the others in the extended state. A microtubule stabilizer, paclitaxel, inhibited coiling and induced right-handed twisting of the haptonema in the absence of Ca2+, suggesting changes in the mechanical properties of microtubules. Addition of Ca2+ resulted in the conversion of haptonematal twist into the planar bends near the proximal region. These results indicate that switching microtubule conformation, possibly with the aid of Ca2+-binding microtubule-associated proteins is responsible for rapid haptonematal coiling. | |||||||||||
書誌情報 |
Biology open 巻 8, 号 2, p. bio036590, 発行日 2019-02 |
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ISSN | ||||||||||||
収録物識別子 | 2046-6390 | |||||||||||
PubMed番号 | ||||||||||||
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関連識別子 | 30700402 | |||||||||||
DOI | ||||||||||||
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関連識別子 | 10.1242/bio.036590 | |||||||||||
権利 | ||||||||||||
権利情報 | © 2019. Published by The Company of Biologists Ltd | |||||||||||
権利 | ||||||||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. | |||||||||||
著者版フラグ | ||||||||||||
値 | publisher | |||||||||||
出版者 | ||||||||||||
出版者 | Company of Biologists |