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  1. 生命環境系 (Faculty of Life and Environmental Sciences)
  2. 中山 剛 (Nakayama Takeshi)
  1. 生命環境系 (Faculty of Life and Environmental Sciences)
  2. 柴 小菊 (Shiba Kogiku)
  1. 生命環境系 (Faculty of Life and Environmental Sciences)
  2. 石田 健一郎 (ISHIDA Ken-ichiro)
  1. 生命環境系 (Faculty of Life and Environmental Sciences)
  2. 稲葉 一男 (Inaba Kazuo)
  1. コンテンツタイプ (Contents Type)
  2. 雑誌発表論文等 (Journal article, etc.)
  3. B~
  4. Biology open

Microtubule stabilizer reveals requirement of Ca2+-dependent conformational changes of microtubules for rapid coiling of haptonema in haptophyte algae

http://hdl.handle.net/2241/00155465
a912517a-bcbd-4084-b37b-2d63675e11ae
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BO_8-2.pdf BO_8-2 (3.6 MB)
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item type Journal Article(1)
公開日 2019-05-17
タイトル
タイトル Microtubule stabilizer reveals requirement of Ca2+-dependent conformational changes of microtubules for rapid coiling of haptonema in haptophyte algae
言語
言語 eng
資源タイプ
タイプ journal article
著者 柴, 小菊

× 柴, 小菊

WEKO 204382
e-Rad 70533561
筑波大学研究者総覧 0000001181

柴, 小菊

ja-Kana シバ, コギク

en SHIBA, Kogiku

Search repository
中山, 剛

× 中山, 剛

WEKO 204614
e-Rad 40302369
筑波大学研究者総覧 0000001161

中山, 剛

ja-Kana ナカヤマ, タケシ

en NAKAYAMA, Takeshi

Search repository
石田, 健一郎

× 石田, 健一郎

WEKO 104
e-Rad 30282198
筑波大学研究者総覧 0000001146

石田, 健一郎

ja-Kana イシダ, ケンイチロウ

en ISHIDA, Ken-ichiro

Search repository
稲葉, 一男

× 稲葉, 一男

WEKO 139945
e-Rad 80221779
筑波大学研究者総覧 0000001201

稲葉, 一男

ja-Kana イナバ, カズオ

en INABA, Kazuo

Search repository
Nomura, Mami

× Nomura, Mami

WEKO 209242

en Nomura, Mami

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Atsuji, Kohei

× Atsuji, Kohei

WEKO 209243

en Atsuji, Kohei

Search repository
Hirose, Keiko

× Hirose, Keiko

WEKO 209244

en Hirose, Keiko

Search repository
Yanase, Ryuji

× Yanase, Ryuji

WEKO 209245

en Yanase, Ryuji

Search repository
抄録
内容記述 A haptonema is an elongated microtubule-based motile organelle uniquely present in haptophytes. The most notable and rapid movement of a haptonema is ‘coiling’, which occurs within a few milliseconds following mechanical stimulation in an unknown motor-independent mechanism. Here, we analyzed the coiling process in detail by high-speed filming and showed that haptonema coiling was initiated by left-handed twisting of the haptonema, followed by writhing to form a helix from the distal tip. On recovery from a mechanical stimulus, the helix slowly uncoiled from the proximal region. Electron microscopy showed that the seven microtubules in a haptonema were arranged mostly in parallel but that one of the microtubules often wound around the others in the extended state. A microtubule stabilizer, paclitaxel, inhibited coiling and induced right-handed twisting of the haptonema in the absence of Ca2+, suggesting changes in the mechanical properties of microtubules. Addition of Ca2+ resulted in the conversion of haptonematal twist into the planar bends near the proximal region. These results indicate that switching microtubule conformation, possibly with the aid of Ca2+-binding microtubule-associated proteins is responsible for rapid haptonematal coiling.
書誌情報 Biology open

巻 8, 号 2, p. bio036590, 発行日 2019-02
ISSN
収録物識別子 2046-6390
PubMed番号
関連識別子
関連識別子 30700402
DOI
関連識別子
関連識別子 10.1242/bio.036590
権利
権利情報 © 2019. Published by The Company of Biologists Ltd
権利
権利情報 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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出版者
出版者 Company of Biologists
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