{"_buckets": {"deposit": "81ab88b4-b3d2-4ce8-9f9e-18897027354d"}, "_deposit": {"id": "46463", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "46463"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00046463"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-03", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "5239", "bibliographicVolumeNumber": "8", "bibliographic_titles": [{"bibliographic_title": "Scientific reports"}]}]}, "item_5_creator_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u7a32\u57a3, \u7950\u53f8"}], "nameIdentifiers": [{"nameIdentifier": "118028", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "50387958", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000050387958"}, {"nameIdentifier": "0000001144", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001144"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Rheb is a conserved and widespread Ras-like GTPase involved in cell growth regulation mediated by the (m)TORC1 kinase complex and implicated in tumourigenesis in humans. Rheb function depends on its association with membranes via prenylated C-terminus, a mechanism shared with many other eukaryotic GTPases. Strikingly, our analysis of a phylogenetically rich sample of Rheb sequences revealed that in multiple lineages this canonical and ancestral membrane attachment mode has been variously altered. The modifications include: (1) accretion to the N-terminus of two different phosphatidylinositol 3-phosphate-binding domains, PX in Cryptista (the fusion being the first proposed synapomorphy of this clade), and FYVE in Euglenozoa and the related undescribed flagellate SRT308; (2) acquisition of lipidic modifications of the N-terminal region, namely myristoylation and/or S-palmitoylation in seven different protist lineages; (3) acquisition of S-palmitoylation in the hypervariable C-terminal region of Rheb in apusomonads, convergently to some other Ras family proteins; (4) replacement of the C-terminal prenylation motif with four transmembrane segments in a novel Rheb paralog in the SAR clade; (5) loss of an evident C-terminal membrane attachment mechanism in Tremellomycetes and some Rheb paralogs of Euglenozoa. Rheb evolution is thus surprisingly dynamic and presents a spectacular example of molecular tinkering.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Nature Publishing Group"}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "29588502", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1038/s41598-018-23575-0", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 The Author(s) 2018"}, {"subitem_rights": "This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article\u2019s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/."}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2045-2322", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Z\u00e1honov\u00e1, Krist\u00edna"}], "nameIdentifiers": [{"nameIdentifier": "189505", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Petr\u017eelkov\u00e1, Romana"}], "nameIdentifiers": [{"nameIdentifier": "189506", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Valach, Matus"}], "nameIdentifiers": [{"nameIdentifier": "189507", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yazaki, Euki"}], "nameIdentifiers": [{"nameIdentifier": "189508", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tikhonenkov, Denis V."}], "nameIdentifiers": [{"nameIdentifier": "189509", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Butenko, Anzhelika"}], "nameIdentifiers": [{"nameIdentifier": "189510", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Janou\u0161kovec, Jan"}], "nameIdentifiers": [{"nameIdentifier": "189511", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hrd\u00e1, \u0160t\u011bp\u00e1nka"}], "nameIdentifiers": [{"nameIdentifier": "189512", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Klime\u0161, Vladim\u00edr"}], "nameIdentifiers": [{"nameIdentifier": "189513", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Burger, Gertraud"}], "nameIdentifiers": [{"nameIdentifier": "189514", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inagaki, Yuji"}], "nameIdentifiers": [{"nameIdentifier": "189515", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Keeling, Patrick J."}], "nameIdentifiers": [{"nameIdentifier": "189516", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hampl, Vladim\u00edr"}], "nameIdentifiers": [{"nameIdentifier": "189517", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Flegontov, Pavel"}], "nameIdentifiers": [{"nameIdentifier": "189518", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yurchenko, Vyacheslav"}], "nameIdentifiers": [{"nameIdentifier": "189519", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Eli\u00e1\u0161, Marek"}], "nameIdentifiers": [{"nameIdentifier": "189520", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-05-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "SR_8.pdf", "filesize": [{"value": "3.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_6", "mimetype": "application/pdf", "size": 3800000.0, "url": {"label": "SR_8-5239", "url": "https://tsukuba.repo.nii.ac.jp/record/46463/files/SR_8.pdf"}, "version_id": "8b2fc703-a9ee-4b57-88e2-adb991c4b99f"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase"}]}, "item_type_id": "5", "owner": "1", "path": ["3048/3118", "3/62/5601/1884"], "permalink_uri": "http://hdl.handle.net/2241/00151743", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2018-05-21"}, "publish_date": "2018-05-21", "publish_status": "0", "recid": "46463", "relation": {}, "relation_version_is_last": true, "title": ["Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase"], "weko_shared_id": 5}