{"_buckets": {"deposit": "3c441632-ec17-4129-8154-903fc3d4f79a"}, "_deposit": {"id": "44461", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "44461"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00044461"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "2312", "bibliographicPageStart": "2304", "bibliographicVolumeNumber": "21", "bibliographic_titles": [{"bibliographic_title": "Cell reports"}]}]}, "item_5_creator_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u65b0\u4e95, \u54f2\u660e"}], "nameIdentifiers": [{"nameIdentifier": "544", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90291145", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000090291145"}, {"nameIdentifier": "0000001819", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001819"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "In the process of drug development, in vitro studies do not always adequately predict human-specific drug responsiveness in clinical trials. Here, we applied the advantage of human iPSC-derived neurons, which offer human-specific drug responsiveness, to screen and evaluate therapeutic candidates for Alzheimer\u2019s disease (AD). Using AD patient neurons with nearly 100% purity from iPSCs, we established a robust and reproducible assay for amyloid \u03b2 peptide (A\u03b2), a pathogenic molecule in AD, and screened a pharmaceutical compound library. We acquired 27 A\u03b2-lowering screen hits, prioritized hits by chemical structure-based clustering, and selected 6 leading compounds. Next, to maximize the anti-A\u03b2 effect, we selected a synergistic combination of bromocriptine, cromolyn, and topiramate as an anti-A\u03b2 cocktail. Finally, using neurons from familial and sporadic AD patients, we found that the cocktail showed a significant and potent anti-A\u03b2 effect on patient cells. This human iPSC-based platform promises to be useful for AD drug development.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier (Cell Press) "}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "29166618", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.celrep.2017.10.109", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2017 The Author(s). "}, {"subitem_rights": "This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)."}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2211-1247", "subitem_source_identifier_type": "ISSN"}]}, "item_5_subject_20": {"attribute_name": "NII\u30b5\u30d6\u30b8\u30a7\u30af\u30c8", "attribute_value_mlt": [{"subitem_subject": "\u533b\u5b66", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kondo, Takayuki"}], "nameIdentifiers": [{"nameIdentifier": "177905", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imamura, Keiko"}], "nameIdentifiers": [{"nameIdentifier": "177906", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Funayama, Misato"}], "nameIdentifiers": [{"nameIdentifier": "177907", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsukita, Kayoko"}], "nameIdentifiers": [{"nameIdentifier": "177908", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miyake, Michiyo"}], "nameIdentifiers": [{"nameIdentifier": "177909", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohta, Akira"}], "nameIdentifiers": [{"nameIdentifier": "177910", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Woltjen, Knut"}], "nameIdentifiers": [{"nameIdentifier": "177911", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakagawa, Masato"}], "nameIdentifiers": [{"nameIdentifier": "177912", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Asada, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "177913", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Arai, Tetsuaki"}], "nameIdentifiers": [{"nameIdentifier": "177914", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawakatsu, Shinobu"}], "nameIdentifiers": [{"nameIdentifier": "177915", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Izumi, Yuishin"}], "nameIdentifiers": [{"nameIdentifier": "177916", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kaji, Ryuji"}], "nameIdentifiers": [{"nameIdentifier": "177917", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iwata, Nobuhisa"}], "nameIdentifiers": [{"nameIdentifier": "177918", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inoue, Haruhisa"}], "nameIdentifiers": [{"nameIdentifier": "177919", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-12-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "CR_21-8.pdf", "filesize": [{"value": "7.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 7800000.0, "url": {"label": "CR_21-8", "url": "https://tsukuba.repo.nii.ac.jp/record/44461/files/CR_21-8.pdf"}, "version_id": "c6bc3fdb-fe9c-4db6-9cf0-a01d45de9f87"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "iPSC-Based Compound Screening and  In Vitro  Trials Identify a Synergistic Anti-amyloid \u03b2 Combination for Alzheimer\u2019s Disease", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "iPSC-Based Compound Screening and  In Vitro  Trials Identify a Synergistic Anti-amyloid \u03b2 Combination for Alzheimer\u2019s Disease"}]}, "item_type_id": "5", "owner": "1", "path": ["2780/1498", "3/62/5298/5294"], "permalink_uri": "http://hdl.handle.net/2241/00149338", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2017-12-15"}, "publish_date": "2017-12-15", "publish_status": "0", "recid": "44461", "relation": {}, "relation_version_is_last": true, "title": ["iPSC-Based Compound Screening and  In Vitro  Trials Identify a Synergistic Anti-amyloid \u03b2 Combination for Alzheimer\u2019s Disease"], "weko_shared_id": 5}