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Simple generation of hairless mice for in vivo imaging
http://hdl.handle.net/2241/00149203
e140ed52-4370-44f5-94b6-179f97e2369d
名前 / ファイル | ライセンス | Actions | |
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item type | Journal Article(1) | |||||
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公開日 | 2017-12-05 | |||||
タイトル | ||||||
タイトル | Simple generation of hairless mice for in vivo imaging | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
タイプ | journal article | |||||
著者 |
Hoshino, Yoshikazu
× Hoshino, Yoshikazu× Mizuno, Seiya× Kato, Kanako× Mizuno-Iijima, Saori× Tanimoto, Yoko× Ishida, Miyuki× Kajiwara, Noriko× Sakasai, Tomoki× Miwa, Yoshihiro× Takahashi, Satoru× Yagami, Ken-ichi× Sugiyama, Fumihiro |
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著者別名 |
高橋, 智
× 高橋, 智× 杉山, 文博× 三輪, 佳宏× 水野, 聖哉 |
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抄録 | ||||||
内容記述 | The in vivo imaging of mice makes it possible to analyze disease progress non-invasively through reporter gene expression. As the removal of hair improves the accuracy of in vivo imaging, gene-modified mice with a reporter gene are often crossed with Hos:HR-1 mutant mice homozygous for the spontaneous Hrhr mutation that exhibit a hair loss phenotype. However, it is time consuming to produce mice carrying both the reporter gene and mutant Hrhr gene by mating. In addition, there is a risk that genetic background of the gene-modified mice would be altered by mating. To resolve these issues, we established a simple method to generate hairless mice maintaining the original genetic background by CRISPR technology. First, we constructed the pX330 vector, which targets exon 3 of Hr. This DNA vector (5 ng/µl) was microinjected into the pronuclei of C57BL/6J mice. Induced Hr gene mutations were found in many founders (76.1%) and these mutations were heritable. Next, we performed in vivo imaging using these gene-modified hairless mice. As expected, luminescent objects in their body were detected by in vivo imaging. This study clearly showed that hairless mice could be simply generated by the CRISPR/Cas9 system, and this method may be useful for in vivo imaging studies with various gene-modified mice. | |||||
書誌情報 |
Experimental Animals 巻 66, 号 4, p. 437-445, 発行日 2017-10 |
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ISSN | ||||||
収録物識別子 | 1341-1357 | |||||
書誌レコードID | ||||||
収録物識別子 | AA11032321 | |||||
PubMed番号 | ||||||
関連識別子 | ||||||
関連識別子 | 28717054 | |||||
DOI | ||||||
関連識別子 | ||||||
関連識別子 | 10.1538/expanim.17-0049 | |||||
権利 | ||||||
権利情報 | © 2017 Japanese Association for Laboratory Animal Science | |||||
著者版フラグ | ||||||
値 | publisher |