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  1. 医学医療系 (Faculty of Medicine)
  2. 杉山 文博 (Sugiyama Fumihiro)
  1. 医学医療系 (Faculty of Medicine)
  2. 水野 聖哉 (Mizuno Seiya)
  1. 医学医療系 (Faculty of Medicine)
  2. 三輪 佳宏 (Miwa Yoshihiro)
  1. 医学医療系 (Faculty of Medicine)
  2. 高橋 智 (Takahashi Satoru)
  1. コンテンツタイプ (Contents Type)
  2. 雑誌発表論文等 (Journal article, etc.)
  3. E~
  4. Experimental animals

Simple generation of hairless mice for in vivo imaging

http://hdl.handle.net/2241/00149203
e140ed52-4370-44f5-94b6-179f97e2369d
プレビュー
名前 / ファイル ライセンス Actions
EA_66-4-437.pdf EA_66-4-437 (2.5 MB)
item type Journal Article(1)
公開日 2017-12-05
タイトル
タイトル Simple generation of hairless mice for in vivo imaging
言語
言語 eng
資源タイプ
タイプ journal article
著者 Hoshino, Yoshikazu

× Hoshino, Yoshikazu

WEKO 177459

Hoshino, Yoshikazu
Search repository
Mizuno, Seiya

× Mizuno, Seiya

WEKO 177460

Mizuno, Seiya
Search repository
Kato, Kanako

× Kato, Kanako

WEKO 177461

Kato, Kanako
Search repository
Mizuno-Iijima, Saori

× Mizuno-Iijima, Saori

WEKO 177462

Mizuno-Iijima, Saori
Search repository
Tanimoto, Yoko

× Tanimoto, Yoko

WEKO 177463

Tanimoto, Yoko
Search repository
Ishida, Miyuki

× Ishida, Miyuki

WEKO 177464

Ishida, Miyuki
Search repository
Kajiwara, Noriko

× Kajiwara, Noriko

WEKO 177465

Kajiwara, Noriko
Search repository
Sakasai, Tomoki

× Sakasai, Tomoki

WEKO 177466

Sakasai, Tomoki
Search repository
Miwa, Yoshihiro

× Miwa, Yoshihiro

WEKO 177467

Miwa, Yoshihiro
Search repository
Takahashi, Satoru

× Takahashi, Satoru

WEKO 177468

Takahashi, Satoru
Search repository
Yagami, Ken-ichi

× Yagami, Ken-ichi

WEKO 177469

Yagami, Ken-ichi
Search repository
Sugiyama, Fumihiro

× Sugiyama, Fumihiro

WEKO 177470

Sugiyama, Fumihiro
Search repository
著者別名 高橋, 智

× 高橋, 智

WEKO 171
e-Rad 50271896
筑波大学研究者総覧 0000001614

高橋, 智
Search repository
杉山, 文博

× 杉山, 文博

WEKO 560
e-Rad 90226481
筑波大学研究者総覧 0000001632

杉山, 文博
Search repository
三輪, 佳宏

× 三輪, 佳宏

WEKO 139951
e-Rad 70263845
筑波大学研究者総覧 0000001677

三輪, 佳宏
Search repository
水野, 聖哉

× 水野, 聖哉

WEKO 204885
e-Rad 10633141
筑波大学研究者総覧 0000003252

水野, 聖哉
Search repository
抄録
内容記述 The in vivo imaging of mice makes it possible to analyze disease progress non-invasively through reporter gene expression. As the removal of hair improves the accuracy of in vivo imaging, gene-modified mice with a reporter gene are often crossed with Hos:HR-1 mutant mice homozygous for the spontaneous Hrhr mutation that exhibit a hair loss phenotype. However, it is time consuming to produce mice carrying both the reporter gene and mutant Hrhr gene by mating. In addition, there is a risk that genetic background of the gene-modified mice would be altered by mating. To resolve these issues, we established a simple method to generate hairless mice maintaining the original genetic background by CRISPR technology. First, we constructed the pX330 vector, which targets exon 3 of Hr. This DNA vector (5 ng/µl) was microinjected into the pronuclei of C57BL/6J mice. Induced Hr gene mutations were found in many founders (76.1%) and these mutations were heritable. Next, we performed in vivo imaging using these gene-modified hairless mice. As expected, luminescent objects in their body were detected by in vivo imaging. This study clearly showed that hairless mice could be simply generated by the CRISPR/Cas9 system, and this method may be useful for in vivo imaging studies with various gene-modified mice.
書誌情報 Experimental Animals

巻 66, 号 4, p. 437-445, 発行日 2017-10
ISSN
収録物識別子 1341-1357
書誌レコードID
収録物識別子 AA11032321
PubMed番号
関連識別子
関連識別子 28717054
DOI
関連識別子
関連識別子 10.1538/expanim.17-0049
権利
権利情報 © 2017 Japanese Association for Laboratory Animal Science
著者版フラグ
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