WEKO3
アイテム
{"_buckets": {"deposit": "ea7c0f80-21ca-44a5-828d-96a83fb1951e"}, "_deposit": {"id": "39534", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "39534"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00039534", "sets": ["306", "1064"]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-10", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "476", "bibliographicPageStart": "471", "bibliographicVolumeNumber": "91", "bibliographic_titles": [{"bibliographic_title": "International journal of biological macromolecules"}]}]}, "item_5_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "白木, 賢太郎"}], "nameIdentifiers": [{"nameIdentifier": "79", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90334797", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000090334797"}, {"nameIdentifier": "0000000710", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000000710"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Arginine is widely used in biotechnological application, but mostly with chloride counter ion. Here, we examined the effects of various anions on solubilization of aromatic compounds and reduced lysozyme and on refolding of the lysozyme. All arginine salts tested increased the solubility of propyl gallate with acetate much more effectively than chloride. The effects of arginine salts were compared with those of sodium or guanidine salts, indicating that the ability of anions to modulate the propyl gallate solubility is independent of the cation. Comparison of transfer free energy of propyl gallate between sodium and arginine salts indicates that the interaction of propyl gallate is more favorable with arginine than sodium. On the contrary, the solubility of aromatic amino acids is only slightly modulated by anions, implying that there is specific interaction between acetic acid and propyl gallate. Unlike their effects on the solubility of small aromatic compounds, the solubility of reduced lysozyme was much higher in arginine chloride than in arginine acetate or sulfate. Consistent with high solubility, refolding of reduced lysozyme was most effective in arginine chloride. These results suggest potential broader applications of arginine modulated by different anions.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "ELSEVIER"}]}, "item_5_relation_10": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "27234496", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.ijbiomac.2016.05.085", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_5_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0141-8130", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00233999", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NIIサブジェクト", "attribute_value_mlt": [{"subitem_subject": "生物学", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yoshizawa, Shunsuke"}], "nameIdentifiers": [{"nameIdentifier": "146929", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Arakawa, Tsutomu"}], "nameIdentifiers": [{"nameIdentifier": "146930", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shiraki, Kentaro"}], "nameIdentifiers": [{"nameIdentifier": "146931", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-11-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "IJBM_91.pdf", "filesize": [{"value": "408.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 408300.0, "url": {"label": "IJBM_91", "url": "https://tsukuba.repo.nii.ac.jp/record/39534/files/IJBM_91.pdf"}, "version_id": "a3186ef0-ff23-4722-98ac-c882cac141db"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Effect of counter ions of arginine as an additive for the solubilization of protein and aromatic compounds", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Effect of counter ions of arginine as an additive for the solubilization of protein and aromatic compounds"}]}, "item_type_id": "5", "owner": "1", "path": ["306", "1064"], "permalink_uri": "http://hdl.handle.net/2241/00144200", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-10-27"}, "publish_date": "2016-10-27", "publish_status": "0", "recid": "39534", "relation": {}, "relation_version_is_last": true, "title": ["Effect of counter ions of arginine as an additive for the solubilization of protein and aromatic compounds"], "weko_shared_id": 5}
Effect of counter ions of arginine as an additive for the solubilization of protein and aromatic compounds
http://hdl.handle.net/2241/00144200
http://hdl.handle.net/2241/00144200a5bb6874-65f2-4398-a699-b9debe2f4085
名前 / ファイル | ライセンス | アクション |
---|---|---|
IJBM_91 (408.3 kB)
|
Item type | Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2016-10-27 | |||||
タイトル | ||||||
タイトル | Effect of counter ions of arginine as an additive for the solubilization of protein and aromatic compounds | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Yoshizawa, Shunsuke
× Yoshizawa, Shunsuke× Arakawa, Tsutomu× Shiraki, Kentaro |
|||||
著者別名 |
白木, 賢太郎
× 白木, 賢太郎 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Arginine is widely used in biotechnological application, but mostly with chloride counter ion. Here, we examined the effects of various anions on solubilization of aromatic compounds and reduced lysozyme and on refolding of the lysozyme. All arginine salts tested increased the solubility of propyl gallate with acetate much more effectively than chloride. The effects of arginine salts were compared with those of sodium or guanidine salts, indicating that the ability of anions to modulate the propyl gallate solubility is independent of the cation. Comparison of transfer free energy of propyl gallate between sodium and arginine salts indicates that the interaction of propyl gallate is more favorable with arginine than sodium. On the contrary, the solubility of aromatic amino acids is only slightly modulated by anions, implying that there is specific interaction between acetic acid and propyl gallate. Unlike their effects on the solubility of small aromatic compounds, the solubility of reduced lysozyme was much higher in arginine chloride than in arginine acetate or sulfate. Consistent with high solubility, refolding of reduced lysozyme was most effective in arginine chloride. These results suggest potential broader applications of arginine modulated by different anions. | |||||
書誌情報 |
International journal of biological macromolecules 巻 91, p. 471-476, 発行日 2016-10 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0141-8130 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00233999 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 27234496 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ijbiomac.2016.05.085 | |||||
権利 | ||||||
権利情報 | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | ELSEVIER |