{"_buckets": {"deposit": "d437a239-18dc-4c6e-b832-eedd8b28cde3"}, "_deposit": {"id": "38418", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "38418"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00038418"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageStart": "e0154285", "bibliographicVolumeNumber": "11", "bibliographic_titles": [{"bibliographic_title": "PLoS ONE"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Objective\nAutoantibodies to melanoma differentiation-associated gene 5 (MDA5) are specifically expressed in patients with dermatomyositis (DM) and are associated with a subset of DM patients with rapidly progressive interstitial lung disease (RP-ILD). Here, we examined the clinical utility of a newly developed enzyme-linked immunosorbent assay (ELISA) system for detecting these antibodies.\nMethods\nHere we developed an improved ELISA for detecting anti-MDA5 antibodies. We then performed a multicenter clinical study involving 8 medical centers and enrolled 242 adult patients with polymyositis (PM)/DM, 190 with non-PM/DM connective tissue disease (CTD), 154 with idiopathic interstitial pneumonia (IIP), and 123 healthy controls. Anti-MDA5 antibodies in the patients\u2019 serum samples were quantified using our newly developed ELISA, and the results were compared to those obtained using the gold-standard immunoprecipitation (IP) assay. In addition, correlations between the ELISA-quantified anti-MDA5 antibodies and clinical characteristics were evaluated.\nResults\nIn patients with PM/DM, the anti-MDA5 antibody measurements obtained from the ELISA and IP assay were highly concordant; the ELISA exhibited an analytical sensitivity of 98.2%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 99.5% (compared to the IP assay). Anti-MDA5 antibodies were detected in 22.7% of the DM patients, but not in any of the patients with PM, non-PM/DM CTD, or IIP. Clinically amyopathic DM, RP-ILD, arthritis, and fever were more prevalent in DM patients who were anti-MDA5 antibody-positive than in those who were antibody-negative (P \u2264 0.0002 for all comparisons). In addition, anti-MDA5 antibody-positive patients with RP-ILD exhibited higher antibody levels than those without RP-ILD (P = 0.006).\nConclusion\nOur newly developed ELISA can detect anti-MDA5 antibodies as efficiently as the gold standard IP assay and has the potential to facilitate the routine clinical measurement of anti-MDA5 antibodies in patients who suspected to have DM.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Public Library of Science"}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "27115353", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1371/journal.pone.0154285", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2016 Sato et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1932-6203", "subitem_source_identifier_type": "ISSN"}]}, "item_5_subject_20": {"attribute_name": "NII\u30b5\u30d6\u30b8\u30a7\u30af\u30c8", "attribute_value_mlt": [{"subitem_subject": " \u533b\u5b66", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sato, Shinji"}], "nameIdentifiers": [{"nameIdentifier": "136703", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murakami, Akihiro"}], "nameIdentifiers": [{"nameIdentifier": "136704", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuwajima, Akiko"}], "nameIdentifiers": [{"nameIdentifier": "136705", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takehara, Kazuhiko"}], "nameIdentifiers": [{"nameIdentifier": "136706", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mimori, Tsuneyo"}], "nameIdentifiers": [{"nameIdentifier": "136707", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawakami, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "136708", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mishima, Michiaki"}], "nameIdentifiers": [{"nameIdentifier": "136709", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suda, Takafumi"}], "nameIdentifiers": [{"nameIdentifier": "136710", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Seishima, Mariko"}], "nameIdentifiers": [{"nameIdentifier": "136711", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujimoto, Manabu"}], "nameIdentifiers": [{"nameIdentifier": "136712", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuwana, Masataka"}], "nameIdentifiers": [{"nameIdentifier": "136713", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-07-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "PO_11-4-e0154285.pdf", "filesize": [{"value": "275.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_6", "mimetype": "application/pdf", "size": 275300.0, "url": {"label": "PO_11-4-e0154285", "url": "https://tsukuba.repo.nii.ac.jp/record/38418/files/PO_11-4-e0154285.pdf"}, "version_id": "8604feb5-e212-4022-a8d5-895c32c4b5ef"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Clinical Utility of an Enzyme-Linked Immunosorbent Assay for Detecting Anti-Melanoma Differentiation-Associated Gene 5 Autoantibodies", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Clinical Utility of an Enzyme-Linked Immunosorbent Assay for Detecting Anti-Melanoma Differentiation-Associated Gene 5 Autoantibodies"}]}, "item_type_id": "5", "owner": "1", "path": ["2780/5228", "3/62/5598/1126"], "permalink_uri": "http://hdl.handle.net/2241/00143086", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2016-07-05"}, "publish_date": "2016-07-05", "publish_status": "0", "recid": "38418", "relation": {}, "relation_version_is_last": true, "title": ["Clinical Utility of an Enzyme-Linked Immunosorbent Assay for Detecting Anti-Melanoma Differentiation-Associated Gene 5 Autoantibodies"], "weko_shared_id": 5}