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Noninvasive Monitoring of β-Cell Mass and Fetal β-Cell Genesis in Mice Using Bioluminescence Imaging
http://hdl.handle.net/2241/118074
888749a8-78f1-44ea-b292-1dd067a6727a
名前 / ファイル | ライセンス | Actions | |
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item type | Journal Article(1) | |||||
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公開日 | 2012-12-19 | |||||
タイトル | ||||||
タイトル | Noninvasive Monitoring of β-Cell Mass and Fetal β-Cell Genesis in Mice Using Bioluminescence Imaging | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
タイプ | journal article | |||||
著者 |
Sekiguchi, Yukari
× Sekiguchi, Yukari× Owada, Junya× Oishi, Hisashi× Katsumata, Tokio× Ikeda, Kaori× Kudo, Takashi× Takahashi, Satoru |
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著者別名 |
大石, 久史
× 大石, 久史× 工藤, 崇× 高橋, 智 |
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抄録 | ||||||
内容記述 | Bioluminescence imaging (BLI) has been applied in gene therapy and research to screen for transgene expression, progression of infection, tumor growth and metastasis, and transplantation. It enables real-time and relatively noninvasive localization and serial quantification of biological processes in experimental animals. In diabetes research, BLI has been employed for the quantification of β-cell mass, monitoring of islet graft survival after transplantation, and detection of reporter gene expression. Here, we explore the use of BLI in a transgenic mouse expressing luciferase under the control of the mouse insulin 1 promoter (MIP-Luc-VU). A previous report on MIP-Luc-VU mice showed luminescence intensities emitted from the islets correlated well with the number of islets in vitro and in vivo. In this study, we showed MIP-Luc-VU mice fed a high fat diet for 8 weeks gave rise to a greater bioluminescent signal than mice fed a regular diet for the same period of time. Conversely, there was a strong reduction in the signal observed in diabetic Mafa-deficient/Mafk-transgenic mutant mice and streptozotocin-treated mice, reflecting the loss of β-cells. Furthermore, we were able to monitor fetal β-cell genesis in MIP-Luc-VU mice during the late gestational stage in a noninvasive and repetitive manner. In summary, we show that bioluminescence imaging of mice expressing a β-cell specific reporter allows detection of changes in β-cell mass and visualization of fetal β-cell neogenesis in uteri. | |||||
書誌情報 |
Experimental animals 巻 61, 号 4, p. 445-451, 発行日 2012 |
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ISSN | ||||||
収録物識別子 | 1341-1357 | |||||
書誌レコードID | ||||||
収録物識別子 | AA11032321 | |||||
PubMed番号 | ||||||
DOI | ||||||
権利 | ||||||
権利情報 | ©2012 Japanese Association for Laboratory Animal Science | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Japanese Association for Laboratory Animal Science |