ログイン
言語:

WEKO3

  • トップ
  • コミュニティ
  • ランキング
AND
To

Field does not validate



インデックスリンク

インデックスツリー

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "8314fd12-1b65-4991-91b5-99da6e4cf6ff"}, "_deposit": {"id": "28087", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28087"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00028087"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "451", "bibliographicPageStart": "445", "bibliographicVolumeNumber": "61", "bibliographic_titles": [{"bibliographic_title": "Experimental animals"}]}]}, "item_5_creator_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5927\u77f3, \u4e45\u53f2"}], "nameIdentifiers": [{"nameIdentifier": "97143", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u5de5\u85e4, \u5d07"}], "nameIdentifiers": [{"nameIdentifier": "115", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20288062", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000020288062"}, {"nameIdentifier": "0000001617", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001617"}]}, {"creatorNames": [{"creatorName": "\u9ad8\u6a4b, \u667a"}], "nameIdentifiers": [{"nameIdentifier": "171", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "50271896", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000050271896"}, {"nameIdentifier": "0000001614", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001614"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Bioluminescence imaging (BLI) has been applied in gene therapy and research to screen for transgene expression, progression of infection, tumor growth and metastasis, and transplantation. It enables real-time and relatively noninvasive localization and serial quantification of biological processes in experimental animals. In diabetes research, BLI has been employed for the quantification of \u03b2-cell mass, monitoring of islet graft survival after transplantation, and detection of reporter gene expression. Here, we explore the use of BLI in a transgenic mouse expressing luciferase under the control of the mouse insulin 1 promoter (MIP-Luc-VU). A previous report on MIP-Luc-VU mice showed luminescence intensities emitted from the islets correlated well with the number of islets in vitro and in vivo. In this study, we showed MIP-Luc-VU mice fed a high fat diet for 8 weeks gave rise to a greater bioluminescent signal than mice fed a regular diet for the same period of time. Conversely, there was a strong reduction in the signal observed in diabetic Mafa-deficient/Mafk-transgenic mutant mice and streptozotocin-treated mice, reflecting the loss of \u03b2-cells. Furthermore, we were able to monitor fetal \u03b2-cell genesis in MIP-Luc-VU mice during the late gestational stage in a noninvasive and repetitive manner. In summary, we show that bioluminescence imaging of mice expressing a \u03b2-cell specific reporter allows detection of changes in \u03b2-cell mass and visualization of fetal \u03b2-cell neogenesis in uteri.", "subitem_description_type": "Abstract"}]}, "item_5_identifier_34": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2241/118074"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Japanese Association for Laboratory Animal Science"}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "22850644", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1538/expanim.61.445", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a92012 Japanese Association for Laboratory Animal Science"}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1341-1357", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11032321", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NII\u30b5\u30d6\u30b8\u30a7\u30af\u30c8", "attribute_value_mlt": [{"subitem_subject": "\u751f\u7269\u5b66", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sekiguchi, Yukari"}], "nameIdentifiers": [{"nameIdentifier": "97136", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Owada, Junya"}], "nameIdentifiers": [{"nameIdentifier": "97137", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oishi, Hisashi"}], "nameIdentifiers": [{"nameIdentifier": "97138", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Katsumata, Tokio"}], "nameIdentifiers": [{"nameIdentifier": "97139", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ikeda, Kaori"}], "nameIdentifiers": [{"nameIdentifier": "97140", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kudo, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "97141", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takahashi, Satoru"}], "nameIdentifiers": [{"nameIdentifier": "97142", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-12-25"}], "displaytype": "detail", "download_preview_message": "", "filename": "EA_61-4.pdf", "filesize": [{"value": "3.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 34000000, "url": {"label": "EA_61-4.pdf", "url": "https://tsukuba.repo.nii.ac.jp/record/28087/files/EA_61-4.pdf"}, "version_id": "efac68eb-9716-4496-a9ca-58ff61b6c4a5"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Noninvasive Monitoring of \u03b2-Cell Mass and Fetal \u03b2-Cell Genesis in Mice Using Bioluminescence Imaging", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Noninvasive Monitoring of \u03b2-Cell Mass and Fetal \u03b2-Cell Genesis in Mice Using Bioluminescence Imaging"}]}, "item_type_id": "5", "owner": "1", "path": ["2780/2186", "2780/542", "2780/544", "3/62/5587/1335"], "permalink_uri": "http://hdl.handle.net/2241/118074", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2012-12-19"}, "publish_date": "2012-12-19", "publish_status": "0", "recid": "28087", "relation": {}, "relation_version_is_last": true, "title": ["Noninvasive Monitoring of \u03b2-Cell Mass and Fetal \u03b2-Cell Genesis in Mice Using Bioluminescence Imaging"], "weko_shared_id": 5}
  1. 医学医療系 (Faculty of Medicine)
  2. 大石 久史 (Oishi Hisashi)
  1. 医学医療系 (Faculty of Medicine)
  2. 工藤 崇 (Kudo Takashi)
  1. 医学医療系 (Faculty of Medicine)
  2. 高橋 智 (Takahashi Satoru)
  1. コンテンツタイプ (Contents Type)
  2. 雑誌発表論文等 (Journal article, etc.)
  3. E~
  4. Experimental animals

Noninvasive Monitoring of β-Cell Mass and Fetal β-Cell Genesis in Mice Using Bioluminescence Imaging

http://hdl.handle.net/2241/118074
888749a8-78f1-44ea-b292-1dd067a6727a
プレビュー
名前 / ファイル ライセンス Actions
EA_61-4.pdf EA_61-4.pdf (3.4 MB)
item type Journal Article(1)
公開日 2012-12-19
タイトル
タイトル Noninvasive Monitoring of β-Cell Mass and Fetal β-Cell Genesis in Mice Using Bioluminescence Imaging
言語
言語 eng
資源タイプ
タイプ journal article
著者 Sekiguchi, Yukari

× Sekiguchi, Yukari

WEKO 97136

Sekiguchi, Yukari

Search repository
Owada, Junya

× Owada, Junya

WEKO 97137

Owada, Junya

Search repository
Oishi, Hisashi

× Oishi, Hisashi

WEKO 97138

Oishi, Hisashi

Search repository
Katsumata, Tokio

× Katsumata, Tokio

WEKO 97139

Katsumata, Tokio

Search repository
Ikeda, Kaori

× Ikeda, Kaori

WEKO 97140

Ikeda, Kaori

Search repository
Kudo, Takashi

× Kudo, Takashi

WEKO 97141

Kudo, Takashi

Search repository
Takahashi, Satoru

× Takahashi, Satoru

WEKO 97142

Takahashi, Satoru

Search repository
著者別名 大石, 久史

× 大石, 久史

WEKO 97143

大石, 久史

Search repository
工藤, 崇

× 工藤, 崇

WEKO 115
e-Rad 20288062
筑波大学研究者総覧 0000001617

工藤, 崇

Search repository
高橋, 智

× 高橋, 智

WEKO 171
e-Rad 50271896
筑波大学研究者総覧 0000001614

高橋, 智

Search repository
抄録
内容記述 Bioluminescence imaging (BLI) has been applied in gene therapy and research to screen for transgene expression, progression of infection, tumor growth and metastasis, and transplantation. It enables real-time and relatively noninvasive localization and serial quantification of biological processes in experimental animals. In diabetes research, BLI has been employed for the quantification of β-cell mass, monitoring of islet graft survival after transplantation, and detection of reporter gene expression. Here, we explore the use of BLI in a transgenic mouse expressing luciferase under the control of the mouse insulin 1 promoter (MIP-Luc-VU). A previous report on MIP-Luc-VU mice showed luminescence intensities emitted from the islets correlated well with the number of islets in vitro and in vivo. In this study, we showed MIP-Luc-VU mice fed a high fat diet for 8 weeks gave rise to a greater bioluminescent signal than mice fed a regular diet for the same period of time. Conversely, there was a strong reduction in the signal observed in diabetic Mafa-deficient/Mafk-transgenic mutant mice and streptozotocin-treated mice, reflecting the loss of β-cells. Furthermore, we were able to monitor fetal β-cell genesis in MIP-Luc-VU mice during the late gestational stage in a noninvasive and repetitive manner. In summary, we show that bioluminescence imaging of mice expressing a β-cell specific reporter allows detection of changes in β-cell mass and visualization of fetal β-cell neogenesis in uteri.
書誌情報 Experimental animals

巻 61, 号 4, p. 445-451, 発行日 2012
ISSN
収録物識別子 1341-1357
書誌レコードID
収録物識別子 AA11032321
PubMed番号
DOI
権利
権利情報 ©2012 Japanese Association for Laboratory Animal Science
著者版フラグ
値 publisher
出版者
出版者 Japanese Association for Laboratory Animal Science
Back
0
views
See details
Views

Versions

Ver.1 2021-03-01 16:53:42.015419
Show All versions

Share

Mendeley CiteULike Twitter Facebook Print Addthis

Cite as

Export

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by CERN Data Centre & Invenio


Powered by CERN Data Centre & Invenio