{"_buckets": {"deposit": "8314fd12-1b65-4991-91b5-99da6e4cf6ff"}, "_deposit": {"id": "28087", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28087"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00028087"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "451", "bibliographicPageStart": "445", "bibliographicVolumeNumber": "61", "bibliographic_titles": [{"bibliographic_title": "Experimental animals"}]}]}, "item_5_creator_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5927\u77f3, \u4e45\u53f2"}], "nameIdentifiers": [{"nameIdentifier": "97143", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u5de5\u85e4, \u5d07"}], "nameIdentifiers": [{"nameIdentifier": "115", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20288062", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000020288062"}, {"nameIdentifier": "0000001617", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001617"}]}, {"creatorNames": [{"creatorName": "\u9ad8\u6a4b, \u667a"}], "nameIdentifiers": [{"nameIdentifier": "171", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "50271896", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000050271896"}, {"nameIdentifier": "0000001614", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001614"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Bioluminescence imaging (BLI) has been applied in gene therapy and research to screen for transgene expression, progression of infection, tumor growth and metastasis, and transplantation. It enables real-time and relatively noninvasive localization and serial quantification of biological processes in experimental animals. In diabetes research, BLI has been employed for the quantification of \u03b2-cell mass, monitoring of islet graft survival after transplantation, and detection of reporter gene expression. Here, we explore the use of BLI in a transgenic mouse expressing luciferase under the control of the mouse insulin 1 promoter (MIP-Luc-VU). A previous report on MIP-Luc-VU mice showed luminescence intensities emitted from the islets correlated well with the number of islets in vitro and in vivo. In this study, we showed MIP-Luc-VU mice fed a high fat diet for 8 weeks gave rise to a greater bioluminescent signal than mice fed a regular diet for the same period of time. Conversely, there was a strong reduction in the signal observed in diabetic Mafa-deficient/Mafk-transgenic mutant mice and streptozotocin-treated mice, reflecting the loss of \u03b2-cells. Furthermore, we were able to monitor fetal \u03b2-cell genesis in MIP-Luc-VU mice during the late gestational stage in a noninvasive and repetitive manner. In summary, we show that bioluminescence imaging of mice expressing a \u03b2-cell specific reporter allows detection of changes in \u03b2-cell mass and visualization of fetal \u03b2-cell neogenesis in uteri.", "subitem_description_type": "Abstract"}]}, "item_5_identifier_34": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2241/118074"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Japanese Association for Laboratory Animal Science"}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "22850644", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1538/expanim.61.445", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a92012 Japanese Association for Laboratory Animal Science"}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1341-1357", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11032321", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NII\u30b5\u30d6\u30b8\u30a7\u30af\u30c8", "attribute_value_mlt": [{"subitem_subject": "\u751f\u7269\u5b66", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sekiguchi, Yukari"}], "nameIdentifiers": [{"nameIdentifier": "97136", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Owada, Junya"}], "nameIdentifiers": [{"nameIdentifier": "97137", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oishi, Hisashi"}], "nameIdentifiers": [{"nameIdentifier": "97138", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Katsumata, Tokio"}], "nameIdentifiers": [{"nameIdentifier": "97139", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ikeda, Kaori"}], "nameIdentifiers": [{"nameIdentifier": "97140", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kudo, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "97141", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takahashi, Satoru"}], "nameIdentifiers": [{"nameIdentifier": "97142", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-12-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "EA_61-4.pdf", "filesize": [{"value": "3.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 3400000.0, "url": {"label": "EA_61-4.pdf", "url": "https://tsukuba.repo.nii.ac.jp/record/28087/files/EA_61-4.pdf"}, "version_id": "efac68eb-9716-4496-a9ca-58ff61b6c4a5"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Noninvasive Monitoring of \u03b2-Cell Mass and Fetal \u03b2-Cell Genesis in Mice Using Bioluminescence Imaging", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Noninvasive Monitoring of \u03b2-Cell Mass and Fetal \u03b2-Cell Genesis in Mice Using Bioluminescence Imaging"}]}, "item_type_id": "5", "owner": "1", "path": ["2780/2186", "2780/542", "2780/544", "3/62/5587/1335"], "permalink_uri": "http://hdl.handle.net/2241/118074", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2012-12-19"}, "publish_date": "2012-12-19", "publish_status": "0", "recid": "28087", "relation": {}, "relation_version_is_last": true, "title": ["Noninvasive Monitoring of \u03b2-Cell Mass and Fetal \u03b2-Cell Genesis in Mice Using Bioluminescence Imaging"], "weko_shared_id": 5}