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Capsaicin-enhanced Ribosomal Protein P2 Expression in Human Intestinal Caco-2 Cells
http://hdl.handle.net/2241/114010
http://hdl.handle.net/2241/1140105b2f0290-7ffc-4c66-8901-e958b91f7f57
名前 / ファイル | ライセンス | アクション |
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Cy_47-1.pdf (306.3 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2011-09-27 | |||||
タイトル | ||||||
タイトル | Capsaicin-enhanced Ribosomal Protein P2 Expression in Human Intestinal Caco-2 Cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Han, Jun Kyu
× Han, Jun Kyu× Akutsu, Mitsuaki× Talorete, Terence P. N.× Maekawa, Takaaki× Tanaka, Toshiyuki× Isoda, Hiroko |
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著者別名 |
韓, 畯奎
× 韓, 畯奎× 田中, 俊之× 礒田, 博子 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | On the basis of transepithelial electrical resistance (TER) measurements, we found that capsaicin (100 μM)-treated human intestinal Caco-2 cells show a momentary increase in tight-junction (TJ) permeability (decrease in TER) followed by a complete recovery. We used proteome analysis to search for proteins that are associated with the recovery of TJ permeability in capsaicin-treated Caco-2 cells. A protein with a relative molecular mass of 14 kDa was found to be expressed more highly in capsaicin-treated cells than in nontreated cells. Mass spectrometry and sequence analyses revealed that the protein that is expressed significantly upon capsaicin treatment is the ribosomal protein P2; its cDNA sequence was identical to that found in the human genome database. An increase in the amount of cellular filamentous actin (F-actin) was shown after 8 h of incubation with capsaicin. It has been reported that P2 activates elongation factor 2, which stabilizes F-actin filaments, and that the depolymerization of F-actin is associated with the increase in TJ permeability (decrease in TER). Consequently, these results suggest that P2 plays an important role in the recovery of the TJ permeability in capsaicin-treated human intestinal cells. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Erratum;Cytotechnology Volume 49, Number 1, 77-85, DOI: 10.1007/s10616-005-6709-0 (関連リソースURI) |
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書誌情報 |
Cytotechnology 巻 47, 号 1-3, p. 89-96, 発行日 2005-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0920-9069 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10678299 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 19003048 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s10616-005-3756-5 | |||||
権利 | ||||||
権利情報 | © Springer 2005 The original publication is available at www.springerlink.com |
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著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Springer | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/114010 | |||||
識別子タイプ | HDL |