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Influences of retinal axons on the cultural substrate containing biotin-conjugated chondroitin sulfate in vitro
http://hdl.handle.net/2241/113919
http://hdl.handle.net/2241/113919167d1ceb-f08a-4192-a4c1-c526dee01259
名前 / ファイル | ライセンス | アクション |
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ASI_85-4.pdf (223.3 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2011-09-15 | |||||
タイトル | ||||||
タイトル | Influences of retinal axons on the cultural substrate containing biotin-conjugated chondroitin sulfate in vitro | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Ando, Satoru
× Ando, Satoru× Sugiura, Nobuo× Kimata, Koji× Ichijo, Hiroyuki |
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著者別名 |
一條, 裕之
× 一條, 裕之 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Although chondroitin sulfate (CS) is known to act as an inhibitory axon guidance cue, retinal axons show substantial growth on a culture substrate containing CS. Thus, the question arises as to how retinal axons elongate on CS-containing culture substrates. To elucidate the effects of retinal axons on a substrate containing CS, we synthesized biotinylated CS (biotin-CS) and developed a culture substrate with streptavidin-conjugated biotin-CS (complex between streptavidin and biotin-CS) to culture retinal axons. The effects of retinal axons on the streptavidin–biotin-CS complex were analyzed immunocytochemically using antibodies against CS and streptavidin, which recognize the carbohydrate and protein portions of the complex, respectively. After the axons were cultured on the substrate, areas that were CS-immunonegative but streptavidin-immunopositive were observed on the surface, corresponding to areas with or without axons, respectively. Absence of CS immunostaining was considered to be caused by structural alterations in the carbohydrate chains of the CS under the influence of the axons. | |||||
書誌情報 |
Anatomical science international 巻 85, 号 4, p. 189-193, 発行日 2010-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1447-6959 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11619036 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 20229367 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s12565-010-0076-4 | |||||
権利 | ||||||
権利情報 | © Japanese Association of Anatomists 2010 The original publication is available at www.springerlink.com |
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著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Springer Japan | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/113919 | |||||
識別子タイプ | HDL |