{"_buckets": {"deposit": "adf1eb55-a13b-4350-a5f4-c109e4149672"}, "_deposit": {"id": "18682", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18682"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00018682"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1996-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "441", "bibliographicPageStart": "435", "bibliographicVolumeNumber": "13", "bibliographic_titles": [{"bibliographic_title": "Zoological science"}]}]}, "item_5_creator_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u677e\u7530, \u5b66"}], "nameIdentifiers": [{"nameIdentifier": "67332", "nameIdentifierScheme": "WEKO"}]}]}, "item_5_description_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "We developed new means of measuring the ratio of the short to the long form (S/L ratio) of the mouse prolactin receptor (mPRL-R) cDNA by PCR using a primer common to the two forms and two specific primers. A means of estimating the amount of mPRL-R cDNA by competitive PCR was also established. We confirmed that these procedures were valid, since the S/L ratio of standard DNA was unaltered by one-sided cPCR amplification under the following conditions: the ratio was between 0.1 and 4, and the amount of cDNA was between 10^3 and 10^7 molecules/tube. The result of one-sided cPCR showed that the short form was dominant in the mouse liver, while the long form was dominant in other tissues. In addition, pituitary grafting increased the S/L ratio in the liver, implying that prolactin down-regulated the functional long form of PRL-R and lowered tissue sensitivity to prolactin itself by modifying the post-transcriptional regulation of PRL-R.", "subitem_description_type": "Abstract"}]}, "item_5_identifier_34": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2241/104403"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65e5\u672c\u52d5\u7269\u5b66\u4f1a"}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "8987522", "subitem_relation_type_select": "PMID"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a91996 Zoological Society of Japan"}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0289-0003", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10545874", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NII\u30b5\u30d6\u30b8\u30a7\u30af\u30c8", "attribute_value_mlt": [{"subitem_subject": "\u751f\u7269\u5b66", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Matsuda, Manabu"}], "nameIdentifiers": [{"nameIdentifier": "67330", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mori, Takao"}], "nameIdentifiers": [{"nameIdentifier": "67331", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-12-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ZS_13-3.pdf", "filesize": [{"value": "696.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 696900.0, "url": {"label": "ZS_13-3.pdf", "url": "https://tsukuba.repo.nii.ac.jp/record/18682/files/ZS_13-3.pdf"}, "version_id": "c683a3c5-995c-47cb-bbf1-5de0bc46fb65"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Modification of Prolactin Receptor (PRL-R) Expression by PRL in the Mouse Liver: Estimation of the Ratio of Two Forms of PRL-R mRNAs by \"One-Sided Competitive PCR\"", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Modification of Prolactin Receptor (PRL-R) Expression by PRL in the Mouse Liver: Estimation of the Ratio of Two Forms of PRL-R mRNAs by \"One-Sided Competitive PCR\""}]}, "item_type_id": "5", "owner": "1", "path": ["2780/1289", "3/62/5613/681"], "permalink_uri": "http://hdl.handle.net/2241/104403", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2010-01-26"}, "publish_date": "2010-01-26", "publish_status": "0", "recid": "18682", "relation": {}, "relation_version_is_last": true, "title": ["Modification of Prolactin Receptor (PRL-R) Expression by PRL in the Mouse Liver: Estimation of the Ratio of Two Forms of PRL-R mRNAs by \"One-Sided Competitive PCR\""], "weko_shared_id": null}