{"_buckets": {"deposit": "92b1e2ec-f8bd-4548-98a6-c7d064b7cd99"}, "_deposit": {"id": "18192", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18192"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00018192"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2009-07", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "21", "bibliographicPageEnd": "3579", "bibliographicPageStart": "3573", "bibliographicVolumeNumber": "30", "bibliographic_titles": [{"bibliographic_title": "Biomaterials"}]}]}, "item_5_creator_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u9234\u6728, \u535a\u7ae0"}], "nameIdentifiers": [{"nameIdentifier": "279", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20282337", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000020282337"}, {"nameIdentifier": "0000000780", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000000780"}]}, {"creatorNames": [{"creatorName": "\u798f\u7530, \u6df3\u4e8c"}], "nameIdentifiers": [{"nameIdentifier": "65074", "nameIdentifierScheme": "WEKO"}]}]}, "item_5_description_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}, {"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Adherent cells, cell sheets, and spheroids were harvested noninvasively from a culture surface by means of electrochemical desorption of a self-assembled monolayer (SAM) of alkanethiol. The SAM surface was made adhesive by the covalent bonding of Arg-Gly-Asp (RGD)-peptides to the alkanethiol molecules. The application of a negative electrical potential caused the reductive desorption of the SAM, resulting in the detachment of the cells. Using this approach greater than 90% of adherent cells detached within 5 min. Furthermore, this approach was used to obtain two-dimensional (2D) cell sheets. The detached cell sheets consisted of viable cells, which could be easily attached to other cell sheets in succession to form a multilayered cell sheet. Moreover, spheroids of hepatocytes of a uniform diameter were formed in an array of cylindrical cavities at a density of 280 spheroids/cm2 and were harvested by applying a negative electrical potential. This cell manipulation technology could potentially be a useful tool for the fabrication and assembly of building blocks such as cell sheets and spheroids for regenerative medicine and tissue engineering applications.", "subitem_description_type": "Abstract"}]}, "item_5_identifier_34": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2241/103631"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier Science Ltd."}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "19362363", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.biomaterials.2009.03.045", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2009 Elsevier Ltd"}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0142-9612", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00110092", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NII\u30b5\u30d6\u30b8\u30a7\u30af\u30c8", "attribute_value_mlt": [{"subitem_subject": "\u533b\u5b66", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Inaba, Rina"}], "nameIdentifiers": [{"nameIdentifier": "65069", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Khademhosseini, Ali"}], "nameIdentifiers": [{"nameIdentifier": "65070", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Hiroaki"}], "nameIdentifiers": [{"nameIdentifier": "65071", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukuda, Junji"}], "nameIdentifiers": [{"nameIdentifier": "65072", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-12-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "B_30-21Figure-1.pdf", "filesize": [{"value": "1.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1500000.0, "url": {"label": "B_30-21Figure-1.pdf", "url": "https://tsukuba.repo.nii.ac.jp/record/18192/files/B_30-21Figure-1.pdf"}, "version_id": "70337251-787d-4d67-b634-c8e1286c1516"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-12-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "B_30-21.pdf", "filesize": [{"value": "154.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 154600.0, "url": {"label": "B_30-21.pdf", "url": "https://tsukuba.repo.nii.ac.jp/record/18192/files/B_30-21.pdf"}, "version_id": "bb5af39b-7adb-4283-9aa8-40e5cc8d5799"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Electrochemical desorption of self-assembled monolayers for engineering cellular tissues", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Electrochemical desorption of self-assembled monolayers for engineering cellular tissues"}]}, "item_type_id": "5", "owner": "1", "path": ["117/810", "2871/2873/808", "3/62/5297/569"], "permalink_uri": "http://hdl.handle.net/2241/103631", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2009-09-11"}, "publish_date": "2009-09-11", "publish_status": "0", "recid": "18192", "relation": {}, "relation_version_is_last": true, "title": ["Electrochemical desorption of self-assembled monolayers for engineering cellular tissues"], "weko_shared_id": 5}