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DNA oligonucleotide-assisted genetic manipulation increases transformation and homologous recombination efficiencies: Evidence from gene targeting of Dictyostelium discoideum
http://hdl.handle.net/2241/98578
http://hdl.handle.net/2241/98578e25101b7-55b2-4463-9ebd-9acdfaf4b563
名前 / ファイル | ライセンス | アクション |
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JBT-133_4.pdf (431.7 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2008-04-10 | |||||
タイトル | ||||||
タイトル | DNA oligonucleotide-assisted genetic manipulation increases transformation and homologous recombination efficiencies: Evidence from gene targeting of Dictyostelium discoideum | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Kuwayama, Hidekazu
× Kuwayama, Hidekazu× Yanagida, Toshio× Ueda, Masahiro |
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著者別名 |
桑山, 秀一
× 桑山, 秀一 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Artificial gene alteration by homologous recombination in living cells, termed gene targeting, presents fundamental and considerable knowledge of in vivo gene function. In principle, this method can possibly be applied to any type of genes and transformable cells. However, its success is limited due to a low frequency of homologous recombination between endogenous targeted gene and exogenous transgene. Here, we describe a general gene-targeting method in which co-transformation of DNA oligonucleotides (oligomers) could significantly increase the homologous recombination frequency and transformation efficiency. The oligomers were simply designed such that they were identical to both the ends of the homologous flanking regions of the targeting construct. Using this strategy, both targeted alleles of diploid cells were simultaneously replaced in a single transformation procedure. Thus, the simplicity and versatility of this method applicable to any type of cell may increase the application of gene targeting. |
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書誌情報 |
Journal of biotechnology 巻 133, 号 4, p. 418-423, 発行日 2008-02-29 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0168-1656 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10458361 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 18160166 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jbiotec.2007.11.002 | |||||
権利 | ||||||
権利情報 | © 2007 Elsevier B.V. | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Elsevier B.V. | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/98578 | |||||
識別子タイプ | HDL | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |