{"created":"2021-03-01T06:52:24.906087+00:00","id":15591,"links":{},"metadata":{"_buckets":{"deposit":"595467d2-22ad-4700-847e-3419c5aaa2a7"},"_deposit":{"id":"15591","owners":[],"pid":{"revision_id":0,"type":"depid","value":"15591"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00015591","sets":["160:516","3:62:5592:202"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-02-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"423","bibliographicPageStart":"418","bibliographicVolumeNumber":"133","bibliographic_titles":[{"bibliographic_title":"Journal of biotechnology"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑山, 秀一"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Artificial gene alteration by homologous recombination in living cells, termed gene targeting, presents fundamental and considerable knowledge\nof in vivo gene function. In principle, this method can possibly be applied to any type of genes and transformable cells. However, its success is\nlimited due to a low frequency of homologous recombination between endogenous targeted gene and exogenous transgene. Here, we describe\na general gene-targeting method in which co-transformation of DNA oligonucleotides (oligomers) could significantly increase the homologous\nrecombination frequency and transformation efficiency. The oligomers were simply designed such that they were identical to both the ends of the\nhomologous flanking regions of the targeting construct. Using this strategy, both targeted alleles of diploid cells were simultaneously replaced in a\nsingle transformation procedure. Thus, the simplicity and versatility of this method applicable to any type of cell may increase the application of\ngene targeting.","subitem_description_type":"Abstract"}]},"item_5_identifier_34":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2241/98578"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"18160166","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jbiotec.2007.11.002","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2007 Elsevier B.V."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0168-1656","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10458361","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kuwayama, Hidekazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagida, Toshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ueda, Masahiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2013-12-19"}],"displaytype":"detail","filename":"JBT-133_4.pdf","filesize":[{"value":"431.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JBT-133_4.pdf","url":"https://tsukuba.repo.nii.ac.jp/record/15591/files/JBT-133_4.pdf"},"version_id":"682c9660-2ed5-41ec-bb6d-b1f3838009cf"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"DNA oligonucleotide-assisted genetic manipulation increases transformation and homologous recombination efficiencies: Evidence from gene targeting of Dictyostelium discoideum","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DNA oligonucleotide-assisted genetic manipulation increases transformation and homologous recombination efficiencies: Evidence from gene targeting of Dictyostelium discoideum"}]},"item_type_id":"5","owner":"1","path":["516","202"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-04-10"},"publish_date":"2008-04-10","publish_status":"0","recid":"15591","relation_version_is_last":true,"title":["DNA oligonucleotide-assisted genetic manipulation increases transformation and homologous recombination efficiencies: Evidence from gene targeting of Dictyostelium discoideum"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2023-07-07T07:55:25.953204+00:00"}