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Runx3-regulated expression of two Ntrk3 transcript variants in dorsal root ganglion neurons
http://hdl.handle.net/2241/00151561
http://hdl.handle.net/2241/001515611eb408e6-60f9-40bc-887b-1cbd87a417f2
名前 / ファイル | ライセンス | アクション |
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DN_76_3 (7.5 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2018-05-07 | |||||
タイトル | ||||||
タイトル | Runx3-regulated expression of two Ntrk3 transcript variants in dorsal root ganglion neurons | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Ogihara, Yuuki
× Ogihara, Yuuki× Masuda, Tomoyuki× Ozaki, Shigeru× Yoshikawa, Masaaki× Shiga, Takashi |
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著者別名 |
増田, 知之
× 増田, 知之× 志賀, 隆 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Somatosensation is divided into proprioception and cutaneous sensation. Dorsal root ganglion (DRG) neurons project their fibers toward peripheral targets including muscles and skin, and centrally to the spinal cord. Proprioceptive DRG neurons transmit information from muscle spindles and Golgi tendon organs to the spinal cord. We previously showed that Runt‐related transcription factor 3 (Runx3) is expressed in these neurons and their projections to the ventral spinal cord and muscle spindles are lost in Runx3‐deficient (Runx3−/−) mouse embryos. Although Runx3 is likely to contribute to the fate decision and projection of proprioceptive DRG neurons, the precise roles for Runx3 in these phenomena are unknown. To identify genes regulated by Runx3 in embryonic DRGs, we performed microarray analyses using cDNAs isolated from wild‐type and Runx3−/− DRGs of embryonic day (E) 12.5 and selected two transcript variants of the tyrosine kinase receptor C (TrkC) gene. These variants, Ntrk3 variant 1 (Ntrk3‐v1) and variant 2 (Ntrk3‐v2), encode full‐length and truncated receptors of neurotrophin‐3, respectively. Using double in situ hybridization, we found that most of Ntrk3‐v1 mRNA expression in E14.5 DRGs depended on Runx3 but that more than half of Ntrk3‐v2 mRNA one were expressed in a Runx3‐independent manner. Furthermore, our data revealed that the rate of Ntrk3‐v1 and Ntrk3‐v2 colocalization in DRGs changed from E14.5 to E18.5. Together, our data suggest that Runx3 may play a crucial role in the development of DRGs by regulating the expression of Ntrk3 variants and that DRG neurons expressing Ntrk3‐v1 but not Ntrk3‐v2 may differentiate into proprioceptive ones. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 313–322, 2016 | |||||
書誌情報 |
Developmental neurobiology 巻 76, 号 3, p. 313-322, 発行日 2016-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19328451 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12195729 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 26061886 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1002/dneu.22316 | |||||
権利 | ||||||
権利情報 | © 2015 Wiley Periodicals, Inc. | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Wiley |