{"created":"2021-03-01T07:26:11.636138+00:00","id":46285,"links":{},"metadata":{"_buckets":{"deposit":"f21bb5a3-ee2b-4218-bdea-ca9d658a944a"},"_deposit":{"id":"46285","owners":[],"pid":{"revision_id":0,"type":"depid","value":"46285"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00046285","sets":["2780:1270","2780:4839","3:62:5586:4838"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"322","bibliographicPageStart":"313","bibliographicVolumeNumber":"76","bibliographic_titles":[{"bibliographic_title":" Developmental neurobiology "}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"増田, 知之"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"志賀, 隆"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Somatosensation is divided into proprioception and cutaneous sensation. Dorsal root ganglion (DRG) neurons project their fibers toward peripheral targets including muscles and skin, and centrally to the spinal cord. Proprioceptive DRG neurons transmit information from muscle spindles and Golgi tendon organs to the spinal cord. We previously showed that Runt‐related transcription factor 3 (Runx3) is expressed in these neurons and their projections to the ventral spinal cord and muscle spindles are lost in Runx3‐deficient (Runx3−/−) mouse embryos. Although Runx3 is likely to contribute to the fate decision and projection of proprioceptive DRG neurons, the precise roles for Runx3 in these phenomena are unknown. To identify genes regulated by Runx3 in embryonic DRGs, we performed microarray analyses using cDNAs isolated from wild‐type and Runx3−/− DRGs of embryonic day (E) 12.5 and selected two transcript variants of the tyrosine kinase receptor C (TrkC) gene. These variants, Ntrk3 variant 1 (Ntrk3‐v1) and variant 2 (Ntrk3‐v2), encode full‐length and truncated receptors of neurotrophin‐3, respectively. Using double in situ hybridization, we found that most of Ntrk3‐v1 mRNA expression in E14.5 DRGs depended on Runx3 but that more than half of Ntrk3‐v2 mRNA one were expressed in a Runx3‐independent manner. Furthermore, our data revealed that the rate of Ntrk3‐v1 and Ntrk3‐v2 colocalization in DRGs changed from E14.5 to E18.5. Together, our data suggest that Runx3 may play a crucial role in the development of DRGs by regulating the expression of Ntrk3 variants and that DRG neurons expressing Ntrk3‐v1 but not Ntrk3‐v2 may differentiate into proprioceptive ones. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 313–322, 2016 ","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley "}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"26061886","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/dneu.22316","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2015 Wiley Periodicals, Inc."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"19328451","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12195729 ","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ogihara, Yuuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuda, Tomoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ozaki, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshikawa, Masaaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shiga, Takashi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-07"}],"displaytype":"detail","filename":"3.DN_76_3_rev.pdf","filesize":[{"value":"7.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DN_76_3","url":"https://tsukuba.repo.nii.ac.jp/record/46285/files/3.DN_76_3_rev.pdf"},"version_id":"2aa4d67f-2ea4-4711-a98b-8459b90e3edd"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Runx3-regulated expression of two Ntrk3 transcript variants in dorsal root ganglion neurons","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Runx3-regulated expression of two Ntrk3 transcript variants in dorsal root ganglion neurons"}]},"item_type_id":"5","owner":"1","path":["1270","4839","4838"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-07"},"publish_date":"2018-05-07","publish_status":"0","recid":"46285","relation_version_is_last":true,"title":["Runx3-regulated expression of two Ntrk3 transcript variants in dorsal root ganglion neurons"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:18:08.741637+00:00"}