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Lineage-affiliated transcription factors bind the Gata3 Tce1 enhancer to mediate lineage-specific programs
http://hdl.handle.net/2241/00138696
http://hdl.handle.net/2241/001386967a66eb89-aefb-4ed9-9c6a-bce42f15c2aa
名前 / ファイル | ライセンス | アクション |
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JCI_126-3.pdf (1.7 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2016-04-21 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Lineage-affiliated transcription factors bind the Gata3 Tce1 enhancer to mediate lineage-specific programs | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
Ohmura, Sakie
× Ohmura, Sakie× 水野, 聖哉× 大石, 久史× Ku, Chia-Jui× Hermann, Mary× Hosoya, Tomonori× 高橋, 智× Engel, James Douglas |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The transcription factor GATA3 is essential for the genesis and maturation of the T cell lineage, and GATA3 dysregulation has pathological consequences. Previous studies have shown that GATA3 function in T cell development is regulated by multiple signaling pathways and that the Notch nuclear effector, RBP-J, binds specifically to the Gata3 promoter. We previously identified a T cell?specific Gata3 enhancer (Tce1) lying 280 kb downstream from the structural gene and demonstrated in transgenic mice that Tce1 promoted T lymphocyte?specific transcription of reporter genes throughout T cell development; however, it was not clear if Tce1 is required for Gata3 transcription in vivo. Here, we determined that the canonical Gata3 promoter is insufficient for Gata3 transcriptional activation in T cells in vivo, precluding the possibility that promoter binding by a host of previously implicated transcription factors alone is responsible for Gata3 expression in T cells. Instead, we demonstrated that multiple lineage-affiliated transcription factors bind to Tce1 and that this enhancer confers T lymphocyte?specific Gata3 activation in vivo, as targeted deletion of Tce1 in a mouse model abrogated critical functions of this T cell?regulatory element. Together, our data show that Tce1 is both necessary and sufficient for critical aspects of Gata3 T cell?specific transcriptional activity. | |||||
言語 | en | |||||
書誌情報 |
en : The journal of clinical investigation 巻 126, 号 3, p. 865-878, 発行日 2016-03 |
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ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0021-9738 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00695520 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 26808502 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1172/JCI83894 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | © 2016, American Society for Clinical Investigation | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | The American Society for Clinical Investigation |