{"created":"2021-03-01T07:16:53.944387+00:00","id":37757,"links":{},"metadata":{"_buckets":{"deposit":"0c084541-8ead-4291-8fbb-e610701e3096"},"_deposit":{"created_by":188,"id":"37757","owners":[188],"pid":{"revision_id":0,"type":"depid","value":"37757"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00037757","sets":["2780:2186","2780:4663","2780:544","3:62:5592:675"]},"author_link":["130062","204885","130065","130066","130067","171","130069"],"item_1644910766877":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"878","bibliographicPageStart":"865","bibliographicVolumeNumber":"126","bibliographic_titles":[{"bibliographic_title":"The journal of clinical investigation","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The transcription factor GATA3 is essential for the genesis and maturation of the T cell lineage, and GATA3 dysregulation has pathological consequences. Previous studies have shown that GATA3 function in T cell development is regulated by multiple signaling pathways and that the Notch nuclear effector, RBP-J, binds specifically to the Gata3 promoter. We previously identified a T cell?specific Gata3 enhancer (Tce1) lying 280 kb downstream from the structural gene and demonstrated in transgenic mice that Tce1 promoted T lymphocyte?specific transcription of reporter genes throughout T cell development; however, it was not clear if Tce1 is required for Gata3 transcription in vivo. Here, we determined that the canonical Gata3 promoter is insufficient for Gata3 transcriptional activation in T cells in vivo, precluding the possibility that promoter binding by a host of previously implicated transcription factors alone is responsible for Gata3 expression in T cells. Instead, we demonstrated that multiple lineage-affiliated transcription factors bind to Tce1 and that this enhancer confers T lymphocyte?specific Gata3 activation in vivo, as targeted deletion of Tce1 in a mouse model abrogated critical functions of this T cell?regulatory element. Together, our data show that Tce1 is both necessary and sufficient for critical aspects of Gata3 T cell?specific transcriptional activity.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The American Society for Clinical Investigation","subitem_publisher_language":"en"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"26808502","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1172/JCI83894","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2016, American Society for Clinical Investigation","subitem_rights_language":"en"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9738","subitem_source_identifier_type":"PISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00695520","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ohmura, Sakie","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"水野, 聖哉","creatorNameLang":"ja"},{"creatorName":"ミズノ, セイヤ","creatorNameLang":"ja-Kana"},{"creatorName":"MIZUNO, Seiya","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"大石, 久史","creatorNameLang":"ja"},{"creatorName":"オオイシ, ヒサシ","creatorNameLang":"ja-Kana"},{"creatorName":"OISHI, Hisashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Ku, Chia-Jui","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hermann, Mary","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hosoya, Tomonori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"高橋, 智","creatorNameLang":"ja"},{"creatorName":"タカハシ, サトル","creatorNameLang":"ja-Kana"},{"creatorName":"TAKAHASHI, Satoru","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Engel, James Douglas","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-21"}],"displaytype":"detail","filename":"JCI_126-3.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tsukuba.repo.nii.ac.jp/record/37757/files/JCI_126-3.pdf"},"version_id":"d243eb57-9583-4484-b200-865d2e865ff5"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Lineage-affiliated transcription factors bind the Gata3 Tce1 enhancer to mediate lineage-specific programs","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Lineage-affiliated transcription factors bind the Gata3 Tce1 enhancer to mediate lineage-specific programs","subitem_title_language":"en"}]},"item_type_id":"5","owner":"188","path":["2186","544","4663","675"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-04-21"},"publish_date":"2016-04-21","publish_status":"0","recid":"37757","relation_version_is_last":true,"title":["Lineage-affiliated transcription factors bind the Gata3 Tce1 enhancer to mediate lineage-specific programs"],"weko_creator_id":"188","weko_shared_id":-1},"updated":"2024-01-25T07:45:37.734722+00:00"}