@article{oai:tsukuba.repo.nii.ac.jp:00009313,
 author = {笹倉, 靖徳 and SASAKURA, Yasunori},
 issue = {7},
 journal = {Developmental dynamics},
 month = {Jul},
 note = {Stable transgenesis is a splendid technique that is applicable to the creation of useful marker lines,
enhancer/gene traps, and insertional mutagenesis. Recently, transposon-mediated transformation using a
Tc1/mariner transposable element Minos has been reported in two ascidians: Ciona intestinalis and C.
savignyi. The transposon derived from an insect, Drosophila hydei, has high activity for excision in Ciona
embryos and transposition in their genome. As much as 37% of Minos-injected C. intestinalis transmitted
transposon insertions to the subsequent generation. Minos-mediated germline transgenesis has also been
achieved by means of electroporation method. Minos techniques have been applied to enhancer traps and
insertional mutagenesis in Ciona. For those reasons, Minos offers the high potential for use as a powerful
tool for future genetic studies. This review specifically addresses recent achievements of transformation
techniques in Ciona, as exemplified using the Minos system.},
 pages = {1758--1767},
 title = {Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis},
 volume = {236},
 year = {2007},
 yomi = {ササクラ, ヤスノリ}
}