{"created":"2021-03-01T06:44:19.325813+00:00","id":8841,"links":{},"metadata":{"_buckets":{"deposit":"8432e3dd-32b3-4996-81b2-d7fe8d4e359c"},"_deposit":{"id":"8841","owners":[],"pid":{"revision_id":0,"type":"depid","value":"8841"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00008841","sets":["3:233:239"]},"author_link":["36081"],"item_12_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991","bibliographicIssueDateType":"Issued"}}]},"item_12_date_granted_46":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"1991-03-25"}]},"item_12_degree_grantor_44":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"筑波大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"University of 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filter)を用いる代わりにカラーカメラの映像を構成する三原色成分の画像を利用することも本法のユニークな点である．本研究では，まず，今回考案した画像解析法の理論的検討を行った．その要点は，分光測光法の最大の問題である分布誤差と非特異的光喪失への対処方法にある．すなわち，1)顕微分光測光法では，分布誤差を減ずるために測定領域を小さな測光スポットで走査する方法が用いられている．画像解析法では，顕微鏡を介して撮影された画像を用いて測定を行うが、画像は画素(pixel)と呼ばれる小さな区画の集合であり，測定対象は入力された時点分割（ディジタル化）されるので，走査の必要がない．顕微分光測光法が｢点」での測定に対して，画像解析法では｢面｣での測定となるため分布誤差の減少，測定精度の向上が期待でき，また，測定時間の短縮が図れる．2)非特異的光喪失に関しては，分光測光法では通常，干渉フィルターを用いた二波長計測することで対処している．本画像解析法においては撮像装置がカラー対応であることに着目し，より簡便に非特異的光喪失を除去する方法として，カラー画像を構成する二成分を干渉フィルターの代わりに利用する方法を考察し，それが理論的に可能であることを示した．(第2章）次に，この細胞核DNAのカラー画像解析法の理論に基づいて実際の測定を行うために必要な装置の構成及び，測定解析のアルゴリズム・手順を具体的に検討した．その際，重要な点はカラーカメラとして自己走査型荷電結合素子(CCD;Charge-Coupled Device)カメラを用い，測定解析アルゴリズムを一般に普及している汎用画像処理装置で稼動できるものとした点である．引き続いて，実際に非特異的喪失除去するための係数γを決定し，各種細胞核DNA量の測定を行い，本画像解析法の定量性・精度を検討した．具体的には，1)本測定法の系統誤差を評価するために，同じDNA量を持ちながら，核の形態・面積が異なるリンパ球と好中球のDNA量を測定し，両者の値を比較した．2)本法の定量性を検討するために，リンパ球の核DNA量とその半分のDNA量を持つDNA量を測定した．3)光波長フィルター(カラーカメラの色成分)を利用したことの妥当性を調べるために，干渉フィルター(band-pass fitlter)を用いて測定した核DNA量と，本法の測定結果とを比較した.(第3章)本法の基礎的な応用としては，種々の培養癌細胞のDNAヒストグラム解析を行い，フローサイトメトリーによって得られたデータと比較した．具体的には，薬剤により細胞周期を同調させた時，及び，培養血清濃度を変化させた時のDNAヒストグラムの変化を解析した．(第4章)最後に，本法の臨床応用として，肺癌の細胞診標本のDNAヒストグラムの解析を行うとともに，画像解析法の特徴を活かして核の形態量の同時計測を試み，核の病理形態学的異型度と比較検討した．(第5章)","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_12_description_45":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"1990","subitem_description_type":"Other"}]},"item_12_dissertation_number_47":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第916号"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"堀口, 尚","creatorNameLang":"ja"},{"creatorName":"Horiguchi, 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