WEKO3
アイテム
{"_buckets": {"deposit": "5c6a7503-2c20-4aae-93eb-a79b9dc2b219"}, "_deposit": {"id": "57091", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "57091"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00057091", "sets": ["7932", "8323"]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "750", "bibliographicPageStart": "739", "bibliographicVolumeNumber": "11", "bibliographic_titles": [{}, {"bibliographic_title": "Methods in Ecology and Evolution", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "1. Ocean acidification by anthropogenic carbon dioxide emissions is projected to depress metabolic and physiological activity in marine calcifiers. To evaluate the sensitivity of marine organisms against ocean acidification, the assimilation of nutrients into carbonate shells and soft tissues must be examined.\n2. We designed a novel experimental protocol, reverse radioisotope labelling, to trace partitioning of nutrients within a single bivalve species under ocean acidification conditions. Injecting CO2 gas, free from radiocarbon, can provide a large contrast between carbon dissolved in the water and the one assimilated from atmosphere. By culturing modern aquifer organisms in acidified seawater, we were able to determine differences in the relative contributions of the end members, dissolved inorganic carbon (DIC) in seawater and metabolic CO2, to shell carbonate and soft tissues.\n3. Under all pCO2 conditions (463, 653, 872, 1,137 and 1,337 μatm), radiocarbon (Δ14C) values of the bivalve Scapharca broughtonii shell were significantly correlated with seawater DIC values; therefore, shell carbonate was derived principally from seawater DIC. The Δ14C results together with stable carbon isotope (δ13C) data suggest that in S. broughtonii shell δ13C may reflect the kinetics of isotopic equilibration as well as end‐member contributions; thus, care must be taken when analysing end‐member contributions by a previous method using δ13C. The insensitivity of S. broughtonii to perturbations in pCO2 up to at least 1,337 µatm indicates that this species can withstand ocean acidification.\n4. Usage of radioisotope to dope for tracer experiments requires strict rules to conduct any operations. Yet, reverse radioisotope labelling proposing in this study has a large advantage and is a powerful tool to understanding physiology of aquifer organisms that can be applicable to various organisms and culture experiments, such as temperature, salinity and acidification experiments, to improve understanding of the proportions of nutrients taken in by marine organisms under changing environments.\n", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "British Ecological Sciety"}, {"subitem_publisher": "John Wiley \u0026 Sons"}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1111/2041-210X.13396", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2020 The Authors. Methods in Ecology and Evolution published by John Wiley \u0026 Sons Ltd on behalf of British Ecological Society This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes."}]}, "item_5_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2041-210X", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "西田, 梢"}, {"creatorName": "ニシダ, コズエ", "creatorNameLang": "ja-Kana"}, {"creatorName": "NISHIDA, Kozue", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "218819", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "0000004403", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000004403"}]}, {"creatorNames": [{"creatorName": "CHEW, Yue Chin", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "225622", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MIYAIRI, Yosuke", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "225623", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HIRABAYASHI, Shoko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "225624", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SUZUKI, Atsushi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "225625", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HAYASHI, Masahiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "225626", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YAMAMOTO, Yuzo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "225627", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SATO, Mizuho", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "225628", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "NOJIRI, Yukihiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "225629", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YOKOYAMA, Yusuke", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "225630", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-02-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "MEE_11-6-739.pdf", "filesize": [{"value": "1.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_9", "mimetype": "application/pdf", "size": 1300000.0, "url": {"label": "MEE_11-6-739", "url": "https://tsukuba.repo.nii.ac.jp/record/57091/files/MEE_11-6-739.pdf"}, "version_id": "4df75f2e-7b5c-4755-928a-4edf272a791a"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Novel reverse radioisotope labelling experiment reveals carbon assimilation of marine calcifiers under ocean acidification conditions", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Novel reverse radioisotope labelling experiment reveals carbon assimilation of marine calcifiers under ocean acidification conditions", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["7932", "8323"], "permalink_uri": "http://hdl.handle.net/2241/00162741", "pubdate": {"attribute_name": "公開日", "attribute_value": "2021-02-16"}, "publish_date": "2021-02-16", "publish_status": "0", "recid": "57091", "relation": {}, "relation_version_is_last": true, "title": ["Novel reverse radioisotope labelling experiment reveals carbon assimilation of marine calcifiers under ocean acidification conditions"], "weko_shared_id": 5}
Novel reverse radioisotope labelling experiment reveals carbon assimilation of marine calcifiers under ocean acidification conditions
http://hdl.handle.net/2241/00162741
http://hdl.handle.net/2241/001627415c873d85-1925-4558-897a-6f91b6b83082
名前 / ファイル | ライセンス | アクション |
---|---|---|
MEE_11-6-739 (1.3 MB)
|
Item type | Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2021-02-16 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Novel reverse radioisotope labelling experiment reveals carbon assimilation of marine calcifiers under ocean acidification conditions | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
西田, 梢
× 西田, 梢× CHEW, Yue Chin× MIYAIRI, Yosuke× HIRABAYASHI, Shoko× SUZUKI, Atsushi× HAYASHI, Masahiro× YAMAMOTO, Yuzo× SATO, Mizuho× NOJIRI, Yukihiro× YOKOYAMA, Yusuke |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 1. Ocean acidification by anthropogenic carbon dioxide emissions is projected to depress metabolic and physiological activity in marine calcifiers. To evaluate the sensitivity of marine organisms against ocean acidification, the assimilation of nutrients into carbonate shells and soft tissues must be examined. 2. We designed a novel experimental protocol, reverse radioisotope labelling, to trace partitioning of nutrients within a single bivalve species under ocean acidification conditions. Injecting CO2 gas, free from radiocarbon, can provide a large contrast between carbon dissolved in the water and the one assimilated from atmosphere. By culturing modern aquifer organisms in acidified seawater, we were able to determine differences in the relative contributions of the end members, dissolved inorganic carbon (DIC) in seawater and metabolic CO2, to shell carbonate and soft tissues. 3. Under all pCO2 conditions (463, 653, 872, 1,137 and 1,337 μatm), radiocarbon (Δ14C) values of the bivalve Scapharca broughtonii shell were significantly correlated with seawater DIC values; therefore, shell carbonate was derived principally from seawater DIC. The Δ14C results together with stable carbon isotope (δ13C) data suggest that in S. broughtonii shell δ13C may reflect the kinetics of isotopic equilibration as well as end‐member contributions; thus, care must be taken when analysing end‐member contributions by a previous method using δ13C. The insensitivity of S. broughtonii to perturbations in pCO2 up to at least 1,337 µatm indicates that this species can withstand ocean acidification. 4. Usage of radioisotope to dope for tracer experiments requires strict rules to conduct any operations. Yet, reverse radioisotope labelling proposing in this study has a large advantage and is a powerful tool to understanding physiology of aquifer organisms that can be applicable to various organisms and culture experiments, such as temperature, salinity and acidification experiments, to improve understanding of the proportions of nutrients taken in by marine organisms under changing environments. |
|||||
書誌情報 |
en : Methods in Ecology and Evolution 巻 11, 号 6, p. 739-750, 発行日 2020-06 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2041-210X | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/2041-210X.13396 | |||||
権利 | ||||||
権利情報 | © 2020 The Authors. Methods in Ecology and Evolution published by John Wiley & Sons Ltd on behalf of British Ecological Society This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | British Ecological Sciety | |||||
出版者 | ||||||
出版者 | John Wiley & Sons |