@article{oai:tsukuba.repo.nii.ac.jp:00056177, author = {菅澤, 威仁 and SUGASAWA, Takehito and 川上, 康 and KAWAKAMI, Yasushi and 竹越, 一博 and TAKEKOSHI, Kazuhiro and Kuji, Tomoaki and Aoki, Kai and Yanazawa, Koki and Takenouchi, Akiko and Watanabe, Makoto and Tome, Yoshiya and Takeuchi, Yoshinori and Aita, Yuichi and Yahagi, Naoya and Shishikura, Yasuhiro and Ono, Seiko and Yoshida, Yasuko}, issue = {3}, journal = {Biomedicines}, month = {Mar}, note = {The aim of this study was to clarify degradation characteristics in each tissue of the knee complex of a medial meniscectomy (MMx)-induced knee osteoarthritis (KOA) animal model using classical methods and an alternative comprehensive evaluation method called contrast-enhanced X-ray micro-computed tomography (CEX-μCT), which was developed in the study. Surgical MMx was performed in the right knee joints of five male Wistar rats to induce KOA. At four weeks post-surgery, the synovitis was evaluated using quantitative polymerase chain reaction (qPCR). Degradations of the articular cartilage of the tibial plateau were evaluated using classical methods and CEX-μCT. Evaluation of the synovitis demonstrated significantly increased expression levels of inflammation-associated marker genes in MMx-treated knees compared with those in sham-treated knees. Evaluation of the articular cartilage using classical methods showed that MMx fully induced degradation of the cartilage. Evaluation using CEX-μCT showed that local areas of the medial cartilage of the tibial plateau were significantly reduced in MMx-treated knees compared with those in sham-treated knees. On the other hand, total cartilage volumes were significantly increased in MMx-treated knees. On the basis of the findings of this study, the method could be relevant to study new treatments in KOA research.}, title = {Characterization of Osteoarthritis in a Medial Meniscectomy-Induced Animal Model Using Contrast-Enhanced X-ray Microtomography}, volume = {8}, year = {2020}, yomi = {スガサワ, タケヒト and カワカミ, ヤスシ and タケコシ, カズヒロ} }