{"created":"2021-03-01T07:37:04.621981+00:00","id":56020,"links":{},"metadata":{"_buckets":{"deposit":"67709d9b-d525-4c47-b1b3-dea806d8bdec"},"_deposit":{"id":"56020","owners":[],"pid":{"revision_id":0,"type":"depid","value":"56020"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00056020","sets":["2780:2534","3:62:5591:8119"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"95","bibliographicPageStart":"85","bibliographicVolumeNumber":"56","bibliographic_titles":[{},{"bibliographic_title":"In Vitro Cellular & Developmental Biology - Animal","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp–derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tissue Culture Association"}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s11626-019-00412-w","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2019"},{"subitem_rights":"This article is distributed under the terms of the Creative Commons At tribution 4.0 International License (http:/ / creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1071-2690","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11003480","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西村, 健"},{"creatorName":"ニシムラ, ケン","creatorNameLang":"ja-Kana"},{"creatorName":"NISHIMURA, Ken","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Hamada, Atsuko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akagi, Eri","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamasaki, Sachiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakatao, Hirotaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Obayashi, Fumitaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohtaka, Manami","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakanishi, Mahito","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toratani, Shigeaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamoto, Tetsuji","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-09-18"}],"displaytype":"detail","filename":"IVCDBA_56-1.pdf","filesize":[{"value":"2.9 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"IVCDBA_56-1","url":"https://tsukuba.repo.nii.ac.jp/record/56020/files/IVCDBA_56-1.pdf"},"version_id":"85db68cb-03a5-4c60-b8f8-889dd0f526b8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["2534","8119"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-09-18"},"publish_date":"2020-09-18","publish_status":"0","recid":"56020","relation_version_is_last":true,"title":["Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:31:03.447803+00:00"}