@article{oai:tsukuba.repo.nii.ac.jp:00056020,
 author = {西村, 健 and NISHIMURA, Ken and Hamada, Atsuko and Akagi, Eri and Yamasaki, Sachiko and Nakatao, Hirotaka and Obayashi, Fumitaka and Ohtaka, Manami and Nakanishi, Mahito and Toratani, Shigeaki and Okamoto, Tetsuji},
 issue = {1},
 journal = {In Vitro Cellular & Developmental Biology - Animal},
 month = {Jan},
 note = {Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp–derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics.},
 pages = {85--95},
 title = {Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions},
 volume = {56},
 year = {2020},
 yomi = {ニシムラ, ケン}
}