@article{oai:tsukuba.repo.nii.ac.jp:00055775,
 author = {大庭, 良介 and OHNIWA, Ryosuke and 森川, 一也 and MORIKAWA, Kazuya and Gor, Vishal and Takemura, Aya J. and Nishitani, Masami and Higashide, Masato and Medrano Romero, Veronica},
 issue = {4},
 journal = {mBio},
 month = {Jul},
 note = {Staphylococcus aureus is an important human pathogen whose success is largely attributed to its vast arsenal of virulence factors that facilitate its invasion into, and survival within, the human host. The expression of these virulence factors is controlled by the quorum sensing accessory gene regulator (Agr) system. However, a large proportion of clinical S. aureus isolates are consistently found to have a mutationally inactivated Agr system. These mutants have a survival advantage in the host but are considered irreversible mutants. Here we show, for the first time, that a fraction of Agr-negative mutants can revert their Agr activity. By serially passaging Agr-negative strains and screening for phenotypic reversion of hemolysis and subsequent sequencing, we identified two mutational events responsible for reversion: a genetic duplication plus inversion event and a poly(A) tract alteration. Additionally, we demonstrate that one clinical Agr-negative methicillin-resistant S. aureus (MRSA) isolate could reproducibly generate Agr-revertant colonies with a poly(A) tract genetic mechanism. We also show that these revertants activate their Agr system upon phagocytosis. We propose a model in which a minor fraction of Agr-negative S. aureus strains are phase variants that can revert their Agr activity and may act as a cryptic insurance strategy against host-mediated stress.},
 title = {Finding of Agr Phase Variants in Staphylococcus aureus},
 volume = {10},
 year = {2019},
 yomi = {オオニワ, リョウスケ and モリカワ, カズヤ}
}