WEKO3
アイテム
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The redox status of cysteine thiol residues of apolipoprotein E impacts on its lipid interactions
http://hdl.handle.net/2241/00160263
http://hdl.handle.net/2241/00160263803df513-b45a-4349-95a7-ebad4bcbd610
名前 / ファイル | ライセンス | アクション |
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BLC_401-5 (2.3 MB)
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Item type | Journal Article(1) | |||||||||||
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公開日 | 2020-06-30 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | The redox status of cysteine thiol residues of apolipoprotein E impacts on its lipid interactions | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
資源タイプ | ||||||||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
タイプ | journal article | |||||||||||
著者 |
山内, 一由
× 山内, 一由
WEKO
465
× 川上, 康 |
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抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | Redox-mediated modulation of cysteine (Cys) thiols has roles in various pathophysiological functions. We recently found that formation of disulfide-linked complexes of apolipoprotein (apo) E3 prevented apoE3 from irreversible oxidation. In this report, the influence of modification of Cys thiols in apoE2 and apoE3 on interactions with lipids was investigated. The apoE redox status was examined by a band-shift assay using a maleimide compound, and interactions with lipids were evaluated by a kinetic assay using dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and non-denaturing polyacrylamide gel electrophoresis. A reduction in DMPC clearance activity of apoE2 and apoE3 but not apoE4 was observed. Although hydrogen peroxide-induced oxidation decreased the clearance activity of the isoforms, apoE2 showed the greatest residual activity. Both Cys thiol masking and dimerization decreased the activity of apoE2 and apoE3 but not apoE4. In contrast, apoAII preincubation markedly increased the activity (apoE2 > apoE3 > apoE4), in accordance with the formation of apoE-AII and apoAII-E2-AII complexes. ApoAII preincubation also reduced the particle size of apoE-DMPC liposome complexes, especially for apoE2. Redox-mediated modification of Cys thiols of apoE2 or apoE3, especially disulfide bond formation with apoAII, affects lipid metabolism and consequently may be responsible for the diverse isoform specificity of apoE. | |||||||||||
書誌情報 |
en : Biological chemistry 巻 401, 号 5, p. 617-627, 発行日 2020-04 |
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ISSN | ||||||||||||
収録物識別子タイプ | ISSN | |||||||||||
収録物識別子 | 1431-6730 | |||||||||||
書誌レコードID | ||||||||||||
収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AA11099140 | |||||||||||
PubMed番号 | ||||||||||||
識別子タイプ | PMID | |||||||||||
関連識別子 | 31913846 | |||||||||||
DOI | ||||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | 10.1515/hsz-2019-0414 | |||||||||||
権利 | ||||||||||||
権利情報 | The final publication is available at https://doi.org/10.1515/hsz-2019-0414 | |||||||||||
著者版フラグ | ||||||||||||
値 | publisher | |||||||||||
出版者 | ||||||||||||
出版者 | De Gruyter |