{"_buckets": {"deposit": "f70bd2c3-c085-4f84-8ed1-1701af30a3fe"}, "_deposit": {"id": "53853", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "53853"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00053853"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-07", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "18", "bibliographicVolumeNumber": "85", "bibliographic_titles": [{}, {"bibliographic_title": "Applied and Environmental Microbiology", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Here we analyzed the innate fluorescence signature of the single microbial cell, within both clonal and mixed populations of microorganisms. We found that even very similarly shaped cells differ noticeably in their autofluorescence features and that the innate fluorescence signatures change dynamically with growth phases. We demonstrated that machine learning models can be trained with a data set of single-cell innate fluorescence signatures to annotate cells according to their phenotypes and physiological status, for example, distinguishing a wild-type Aspergillus nidulans cell from its nitrogen metabolism mutant counterpart and log-phase cells from stationary-phase cells of Pseudomonas putida We developed a minimally invasive method (confocal reflection microscopy-assisted single-cell innate fluorescence [CRIF] analysis) to optically extract and catalog the innate cellular fluorescence signatures of each of the individual live microbial cells in a three-dimensional space. This technique represents a step forward from traditional techniques which analyze the innate fluorescence signatures at the population level and necessitate a clonal culture. Since the fluorescence signature is an innate property of a cell, our technique allows the prediction of the types or physiological status of intact and tag-free single cells, within a cell population distributed in a three-dimensional space. Our study presents a blueprint for a streamlined cell analysis where one can directly assess the potential phenotype of each single cell in a heterogenous population by its autofluorescence signature under a microscope, without cell tagging.IMPORTANCE A cell\u0027s innate fluorescence signature is an assemblage of fluorescence signals emitted by diverse biomolecules within a cell. It is known that the innate fluoresce signature reflects various cellular properties and physiological statuses; thus, they can serve as a rich source of information in cell characterization as well as cell identification. However, conventional techniques focus on the analysis of the innate fluorescence signatures at the population level but not at the single-cell level and thus necessitate a clonal culture. In the present study, we developed a technique to analyze the innate fluorescence signature of a single microbial cell. Using this novel method, we found that even very similarly shaped cells differ noticeably in their autofluorescence features, and the innate fluorescence signature changes dynamically with growth phases. We also demonstrated that the different cell types can be classified accurately within a mixed population under a microscope at the resolution of a single cell, depending solely on the innate fluorescence signature information. We suggest that single-cell autofluoresce signature analysis is a promising tool to directly assess the taxonomic or physiological heterogeneity within a microbial population, without cell tagging.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "American Society for Microbiology"}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "31324624", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1128/AEM.00608-19", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2019 Yawata et al. "}, {"subitem_rights": "This is anopen-access article distributed under the termsof theCreative Commons Attribution 4.0Internationallicense."}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0099-2240", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00543249", "subitem_source_identifier_type": "NCID"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u516b\u5e61, \u7a63"}, {"creatorName": "\u30e4\u30ef\u30bf, \u30e6\u30bf\u30ab", "creatorNameLang": "ja-Kana"}, {"creatorName": "YAWATA, Yutaka", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "205013", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "10586457", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000010586457"}, {"nameIdentifier": "0000003972", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000003972"}]}, {"creatorNames": [{"creatorName": "\u91ce\u6751, \u66a2\u5f66"}, {"creatorName": "\u30ce\u30e0\u30e9, \u30ce\u30d6\u30d2\u30b3", "creatorNameLang": "ja-Kana"}, {"creatorName": "NOMURA, Nobuhiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "163128", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60292520", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000060292520"}, {"nameIdentifier": "0000001409", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001409"}]}, {"creatorNames": [{"creatorName": "Kiyokawa, Tatsunori", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "216908", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawamura, Yuhki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "216909", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirayama, Tomohiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "216910", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takabe, Kyosuke", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "216911", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-01-09"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AEM_85-18.pdf", "filesize": [{"value": "2.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_6", "mimetype": "application/pdf", "size": 2900000.0, "url": {"label": "AEM_85-18", "url": "https://tsukuba.repo.nii.ac.jp/record/53853/files/AEM_85-18.pdf"}, "version_id": "c55df005-38c1-436c-96ce-58b1f7c7595e"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Intra- and Interspecies Variability of Single-Cell Innate Fluorescence Signature of Microbial Cell", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Intra- and Interspecies Variability of Single-Cell Innate Fluorescence Signature of Microbial Cell", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["160/1253", "160/7596", "3/62/5296/373"], "permalink_uri": "http://hdl.handle.net/2241/00159271", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2020-01-09"}, "publish_date": "2020-01-09", "publish_status": "0", "recid": "53853", "relation": {}, "relation_version_is_last": true, "title": ["Intra- and Interspecies Variability of Single-Cell Innate Fluorescence Signature of Microbial Cell"], "weko_shared_id": 5}