{"_buckets": {"deposit": "3abc4f33-1a1b-4aa2-a552-8f79bf566ed6"}, "_deposit": {"id": "52307", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "52307"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00052307"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "1652", "bibliographicPageStart": "1644", "bibliographicVolumeNumber": "110", "bibliographic_titles": [{"bibliographic_title": "Cancer science"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Cancer tissues contain small populations of highly tumorigenic cells termed cancer stem cells (CSCs). Immortalized cell lines containing CSCs are valuable and powerful experimental tools for research into the characteristics of these stem cells. We previously reported that the hepatocellular carcinoma cell line Li\u20107 includes abundant CD13+CD166\u2212 CSCs; however, the number of these cells decreases after long\u2010term culture as a result of differentiation to non\u2010CSC populations. To ensure consistent and reproducible results in experiments using Li\u20107 cells, it is important that the CSC population is maintained stably regardless of culture duration and passage. In the present study, we found that a commercially available culture medium for maintenance of embryonic stem cells and induced pluripotent stem cells, mTeSR1, effectively prevented spontaneous differentiation by CD13+CD166\u2212 cells to CD13\u2212CD166+ cells and therefore maintained the CSC population in Li\u20107 cell cultures. CD13+CD166\u2212 CSCs maintained using this culture medium retained high tumorigenicity after transplantation into mice; they also showed the ability to differentiate in vitro into non\u2010CSC populations in RPMI\u20101640 with 10% FBS medium. We analyzed gene expression profiles of CSC and non\u2010CSC populations in Li\u20107 cultures using an RNA sequencing method. Genes such as FGFR, NOTCH1, and JAG1, that are associated with tumorigenicity and stemness, were upregulated in the CSC population. Our results suggest that CSCs can be maintained in immortalized cancer cell lines cultured over an extended period using a medium developed for culture of embryonic/induced pluripotent stem cells.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "wiley"}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "30784169", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1111/cas.13978", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. \u00a9 2019 The Authors. Cancer Science published by John Wiley \u0026 Sons Australia, Ltd on behalf of Japanese Cancer Association."}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1347-9032", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11808050", "subitem_source_identifier_type": "NCID"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5c71\u7530, \u6b66\u53f2"}, {"creatorName": "\u30e4\u30de\u30c0, \u30bf\u30b1\u30b7", "creatorNameLang": "ja-Kana"}, {"creatorName": "YAMADA, Takeshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "204997", 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