@article{oai:tsukuba.repo.nii.ac.jp:00051666,
 author = {宮崎, 均 and MIYAZAKI, Hitoshi and 吉田, 滋樹 and YOSHIDA, Shigeki and Hagiwara, Hiromi and Nakata, Kyoko and Maehashi, Sanae and Komiyama, Yuki and Aida, Rieko and Kokubu, Daichi and Hagiwara, Keitaro and Yoshida, Kaoru},
 issue = {1},
 journal = {Cytotechnology},
 month = {Feb},
 note = {4-Hydroxyderricin (4-HD) is a major polyphenol of Angelica keiskei (Japanese name Ashitaba), exhibiting anti-allergic, anti-diabetic, anti-oxidant, and antitumor effects. The present study was designed to evaluate the effects of 4-HD on bone formation and maintenance by using cultured osteoclasts and osteoblasts. 4-HD did not affect cell proliferation of stromal ST2 cells and preosteoblast MC3T3-E1 cells at concentrations of 1–10 μM. This compound inhibited the formation of multinucleated osteoclasts from mouse splenic cells, and we identified a molecular pathway of osteoclast differentiation mediated by 4-HD, which led to inhibition of the expression of receptor activator of nuclear factor-κB ligand and macrophage-colony stimulating factor in ST2 cells. By contrast, 4-HD enhanced indices of osteoblast differentiation, such as alkaline phosphatase activity and calcium deposition by osteoblastic MC3T3-E1 cells, at concentrations of 1–10 μM. Furthermore, we found that 4-HD at 1 μM attenuated H2O2 levels in MC3T3-E1 cells. Our findings indicate that 4-HD may have critical effects on bone formation and maintenance.},
 pages = {15--22},
 title = {4-Hydroxyderricin inhibits osteoclast formation and accelerates osteoblast differentiation},
 volume = {71},
 year = {2019},
 yomi = {ミヤザキ, ヒトシ and ヨシダ, シゲキ}
}