@article{oai:tsukuba.repo.nii.ac.jp:00049313,
 author = {石井, 亜紀子 and ISHII, Akiko and 玉岡, 晃 and TAMAOKA, Akira and Tomono, Taro and Hirai, Yukihiko and Okada, Hironori and Miyagawa, Yoshitaka and Adachi, Kumi and Sakamoto, Shuhei and Kawano, Yasuhiro and Chono, Hideto and Mineno, Junichi and Shimada, Takashi and Onodera, Masafumi and Okada, Takashi},
 journal = {Molecular Therapy - Methods & Clinical Development},
 month = {Dec},
 note = {Recombinant adeno-associated virus serotype 9 (rAAV9) can specifically transduce muscle and neuronal tissues; thus, rAAV9 can potentially be used in gene therapy. However, rAAV9 is the most challenging rAAV serotype to purify. Traditionally, rAAV9 has been purified by ultracentrifugation, which is not scalable. We recently described a chromatographic purification protocol for rAAV1; this protocol can achieve scalable purifications. In this study, we attempted to optimize this protocol for purifying rAAV9 preparations, and we developed a novel, effective method for high-yield purification of rAAV9 using quaternary ammonium anion exchangers and size-exclusion chromatography. The final purified rAAV9 contained mainly three capsid proteins, as observed by SDS-PAGE. Furthermore, negative-stain electron microscopy demonstrated that 96.1% ± 1.1% of rAAV9 particles carried the viral genome containing the EGFP transgene, indicating that impurities and empty capsids can be eliminated with our purification protocol. The final rAAV9 titer obtained by our protocol totaled 2.5 ± 0.4 × 1015 viral genomes produced from ∼3.2 × 109 HEK293EB cells. We confirmed that our protocol can also be applied to purify other varied AAV genome constructs. Our protocol can scale up production of pure rAAV9, in compliance with current good manufacturing practice, for clinical applications in human gene therapy.},
 pages = {180--190},
 title = {Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9},
 volume = {11},
 year = {2018},
 yomi = {イシイ, アキコ and タマオカ, アキラ}
}