{"created":"2021-03-01T07:29:17.027273+00:00","id":49112,"links":{},"metadata":{"_buckets":{"deposit":"071c9a0e-da73-4066-8125-7e7116ce7582"},"_deposit":{"id":"49112","owners":[],"pid":{"revision_id":0,"type":"depid","value":"49112"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00049112","sets":["160:2679","160:857","3:62:5592:2556"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"2802","bibliographicPageStart":"2793","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Journal of Applied Phycology"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Lipid droplets are ubiquitous cellular compartments that store neutral lipids and specific proteins localize on their surface. These proteins work as a scaffold in maintaining the lipid droplet structure or as regulators of lipogenesis or lipolysis. Previously, the most abundant lipid droplet protein, namely stramenopile-type lipid droplet protein (StLDP), was identified in the marine diatom Phaeodactylum tricornutum; however, its function remains unclear because StLDP does not reveal homology with known lipid droplet proteins and lacks a predictable domain. In this study, P. tricornutum was transformed to express a homologous StLDP gene under an fcpA promoter in order to determine its function. StLDP expression was strongly enhanced in the mutant (H8), especially in nitrogen-sufficient conditions; however, it was attenuated in nitrogen-deficient conditions. Despite the strong expression, no significant difference was observed in the lipid composition between the wild type (WT) and H8 under nitrogen-sufficient conditions. After cultivation in nitrogen-free medium for 6 days, neutral lipid content significantly increased in H8 than in WT. After 2 days of cultivation in nitrogen-free medium, 97.0% of single cells in WT formed one or two lipid droplets, whereas in H8, this proportion decreased to 78.8%, and the proportion of cells forming three or four lipid droplets increased. Thus, the function of StLDP was speculated to sequester triacylglycerol on the initial lipid droplet formation.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Netherlands"}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s10811-018-1402-9","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is a pre-print of an article published in Journal of Applied Phycology. The final authenticated version is available online at: https://doi.org/10.1007/s10811-018-1402-9"}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0921-8971","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10729877 ","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"吉田, 昌樹"},{"creatorName":"ヨシダ, マサキ","creatorNameLang":"ja-Kana"},{"creatorName":"YOSHIDA, Masaki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"鈴木, 石根"},{"creatorName":"スズキ, イワネ","creatorNameLang":"ja-Kana"},{"creatorName":"SUZUKI, Iwane","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Yoneda, Kohei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Makoto M.","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-01"}],"displaytype":"detail","filename":"JAP_30-5-2793.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JAP_30-5-2793","url":"https://tsukuba.repo.nii.ac.jp/record/49112/files/JAP_30-5-2793.pdf"},"version_id":"58e16cd0-9092-45b8-b20e-ed64008561f7"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Homologous expression of lipid droplet protein-enhanced neutral lipid accumulation in the marine diatom Phaeodactylum tricornutum","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Homologous expression of lipid droplet protein-enhanced neutral lipid accumulation in the marine diatom Phaeodactylum tricornutum"}]},"item_type_id":"5","owner":"1","path":["857","2679","2556"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-02-13"},"publish_date":"2019-02-13","publish_status":"0","recid":"49112","relation_version_is_last":true,"title":["Homologous expression of lipid droplet protein-enhanced neutral lipid accumulation in the marine diatom Phaeodactylum tricornutum"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:21:31.925767+00:00"}