{"created":"2021-03-01T07:28:39.993542+00:00","id":48552,"links":{},"metadata":{"_buckets":{"deposit":"977ce4e3-7bc8-4957-a314-aaf718680508"},"_deposit":{"id":"48552","owners":[],"pid":{"revision_id":0,"type":"depid","value":"48552"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00048552","sets":["2780:5301","2780:5369","2780:544","3:62:5601:1884"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-09","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"14515","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"Scientific reports"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"濱田, 理人"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"高橋, 智"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"三輪, 佳宏"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"By using near-infrared fluorescent protein (iRFP)-expressing hematopoietic cells, we established a novel, quantitative, in vivo, noninvasive atherosclerosis imaging system. This murine atherosclerosis imaging approach targets macrophages expressing iRFP in plaques. Low-density lipoprotein receptor-deficient (LDLR−/−) mice transplanted with beta-actin promoter-derived iRFP transgenic (TG) mouse bone marrow (BM) cells (iRFP → LDLR−/−) were used. Atherosclerosis was induced by a nonfluorescent 1.25% cholesterol diet (HCD). Atherosclerosis was compared among the three differently induced mouse groups. iRFP → LDLR−/− mice fed a normal diet (ND) and LDLR−/− mice transplanted with wild-type (WT) BM cells were used as controls. The in vivo imaging system (IVIS) detected an enhanced iRFP signal in the thoracic aorta of HCD-fed iRFP → LDLR−/− mice, whereas iRFP signals were not observed in the control mice. Time-course imaging showed a gradual increase in the signal area, which was correlated with atherosclerotic plaque progression. Oil red O (ORO) staining of aortas and histological analysis of plaques confirmed that the detected signal was strictly emitted from plaque-positive areas of the aorta. Our new murine atherosclerosis imaging system can noninvasively image atherosclerotic plaques in the aorta and generate longitudinal data, validating the ability of the system to monitor lesion progression.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Publishing Group"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"30266983","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-018-32456-5","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2018"},{"subitem_rights":"This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kulathunga, Kaushalya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hamada, Michito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hiraishi, Yukiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Otake, Mao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tran, Mai Thi Nhu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cheng, Olivia"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Junko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakasai, Tomoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakaguchi, Shota"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugiyama, Yuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fleischmann, Bernd K."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Satoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miwa, Yoshihiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-12-04"}],"displaytype":"detail","filename":"SR_8-14515.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"SR_8-14515","url":"https://tsukuba.repo.nii.ac.jp/record/48552/files/SR_8-14515.pdf"},"version_id":"222208dd-fdb1-4ab4-968d-d4ffefe1aa5a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System"}]},"item_type_id":"5","owner":"1","path":["544","5369","5301","1884"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-12-04"},"publish_date":"2018-12-04","publish_status":"0","recid":"48552","relation_version_is_last":true,"title":["A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:20:45.395160+00:00"}