@article{oai:tsukuba.repo.nii.ac.jp:00047720,
 author = {稲葉, 一男 and Hill, Richard W. and Armstrong, Eric J. and Inaba, Kazuo and Morita, Masaya and Tresguerres, Martin and Stillman, Jonathon H. and Roa, Jinae N. and Kwan, Garfield T.},
 issue = {6},
 journal = {Biology letters},
 month = {Jun},
 note = {The giant clam Tridacna crocea, native to Indo-Pacific coral reefs, is noted for its unique ability to bore fully into coral rock and is a major agent of reef bioerosion. However, T. crocea's mechanism of boring has remained a mystery despite decades of research. By exploiting a new, two-dimensional pH-sensing technology and manipulating clams to press their presumptive boring tissue (the pedal mantle) against pH-sensing foils, we show that this tissue lowers the pH of surfaces it contacts by greater than or equal to 2 pH units below seawater pH day and night. Acid secretion is likely mediated by vacuolar-type H+-ATPase, which we demonstrate (by immunofluorescence) is abundant in the pedal mantle outer epithelium. Our discovery of acid secretion solves this decades-old mystery and reveals that, during bioerosion, T. crocea can liberate reef constituents directly to the soluble phase, rather than producing sediment alone as earlier assumed.},
 title = {Acid secretion by the boring organ of the burrowing giant clam, Tridacna crocea},
 volume = {14},
 year = {2018}
}