@article{oai:tsukuba.repo.nii.ac.jp:00045979,
 author = {田原, 聡子 and 渋谷, 彰 and Shibagaki, Shohei and Tahara-Hanaoka, Satoko and Hiroyama, Takashi and Nakamura, Yukio and Shibuya, Akira},
 issue = {5},
 journal = {International immunology},
 month = {May},
 note = {Mast cells (MCs) play pivotal roles in allergic reactions and the host defense against microbial infection through the IgE-dependent and IgE-independent signaling pathways. MC lines that can be analyzed both in vitro and in vivo would be useful for the study of MC-dependent immune responses. Here, we investigated the functional characteristics of a mouse embryonic stem cell-derived MC-like cell line, MEDMC-BRC6. The cell line expressed FcεRI and c-Kit and showed degranulation and production of inflammatory cytokines and chemokines, including TNF-α, IL-6 and MCP-1, upon cross-linking FcεRI with IgE. These cytokines and chemokines were also produced by the cell line by stimulation of TLR2 and TLR4. MEDMC-BRC6 survived in the peritoneal cavity and the ear skin for at least 6 months after the transfer into genetically compatible MC-deficient KitW-sh/W-sh mice, in which systemic anaphylaxis was successfully induced. Thus, MEDMC-BRC6 cells represent a potent tool for investigating the functions of MCs in vitro and in vivo.},
 pages = {235--242},
 title = {Long-term survival of the mouse ES cell-derived mast cell, MEDMC-BRC6, in mast cell-deficient Kit W-sh/W-sh mice},
 volume = {29},
 year = {2017}
}