WEKO3
アイテム
{"_buckets": {"deposit": "936e3089-653f-47b5-bb40-26db1c1c7413"}, "_deposit": {"id": "45441", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "45441"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00045441", "sets": ["5104", "6371"]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-12", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "120", "bibliographicVolumeNumber": "17", "bibliographic_titles": [{"bibliographic_title": "Cancer cell international"}]}]}, "item_5_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "関根, 郁夫"}], "nameIdentifiers": [{"nameIdentifier": "130572", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "10508310", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000010508310"}, {"nameIdentifier": "0000003751", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000003751"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background\nPemetrexed (PEM) is an anti-cancer agent targeting DNA and RNA synthesis, and clinically in use for mesothelioma and non-small cell lung carcinoma. A mechanism of resistance to PEM is associated with elevated activities of several enzymes involved in nucleic acid metabolism.\n\nMethods\nWe established two kinds of PEM-resistant mesothelioma cells which did not show any increase of the relevant enzyme activities. We screened genes enhanced in the PEM-resistant cells with a microarray analysis and confirmed the expression levels with Western blot analysis. A possible involvement of the candidates in the PEM-resistance was examined with a WST assay after knocking down the expression with si-RNA. We also analyzed a mechanism of the up-regulated expression with agents influencing AMP-activated protein kinase (AMPK) and p53.\n\nResults\nWe found that expression of cardiac ankyrin repeat protein (CARP) was elevated in the PEM-resistant cells with a microarray and Western blot analysis. Down-regulation of CARP expression with si-RNA did not however influence the PEM resistance. Parent and PEM-resistant cells treated with PEM increased expression of CARP, AMPK, p53 and histone H2AX. The CARP up-regulation was however irrelevant to the p53 genotypes and not induced by an AMPK activator. Augmented p53 levels with nutlin-3a, an inhibitor for p53 degradation, and DNA damages were not always associated with the enhanced CARP expression.\n\nConclusions\nThese data collectively suggest that up-regulated CARP expression is a potential marker for development of PEM-resistance in mesothelioma and that the PEM-mediated enhanced expression is not directly linked with immediate cellular responses to PEM.\n", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "BioMed Central"}]}, "item_5_relation_10": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "29238267", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1186/s12935-017-0493-8", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/ publicdomain/zero/1.0/ ) applies to the data made available in this article, unless otherwise stated."}]}, "item_5_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1475-2867", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12050420", "subitem_source_identifier_type": "NCID"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Qin, Yiyang"}], "nameIdentifiers": [{"nameIdentifier": "182663", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sekine, Ikuo"}], "nameIdentifiers": [{"nameIdentifier": "182664", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fan, Mengmeng"}], "nameIdentifiers": [{"nameIdentifier": "182665", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takiguchi, Yuichi"}], "nameIdentifiers": [{"nameIdentifier": "182666", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tada, Yuji"}], "nameIdentifiers": [{"nameIdentifier": "182667", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shingyoji, Masato"}], "nameIdentifiers": [{"nameIdentifier": "182668", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hanazono, Michiko"}], "nameIdentifiers": [{"nameIdentifier": "182669", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamaguchi, Naoto"}], "nameIdentifiers": [{"nameIdentifier": "182670", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tagawa, Masatoshi"}], "nameIdentifiers": [{"nameIdentifier": "182671", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-26"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "CCI_17-120.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_6", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "CCI_17-120", "url": "https://tsukuba.repo.nii.ac.jp/record/45441/files/CCI_17-120.pdf"}, "version_id": "078d2b54-d765-4442-abed-84b7988944ec"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells"}]}, "item_type_id": "5", "owner": "1", "path": ["5104", "6371"], "permalink_uri": "http://hdl.handle.net/2241/00150718", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-02-26"}, "publish_date": "2018-02-26", "publish_status": "0", "recid": "45441", "relation": {}, "relation_version_is_last": true, "title": ["Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells"], "weko_shared_id": 5}
Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells
http://hdl.handle.net/2241/00150718
http://hdl.handle.net/2241/00150718ef16f718-cb11-4944-9b00-ed7ce64e20fe
名前 / ファイル | ライセンス | アクション |
---|---|---|
CCI_17-120 (1.7 MB)
|
Item type | Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2018-02-26 | |||||
タイトル | ||||||
タイトル | Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Qin, Yiyang
× Qin, Yiyang× Sekine, Ikuo× Fan, Mengmeng× Takiguchi, Yuichi× Tada, Yuji× Shingyoji, Masato× Hanazono, Michiko× Yamaguchi, Naoto× Tagawa, Masatoshi |
|||||
著者別名 |
関根, 郁夫
× 関根, 郁夫 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background Pemetrexed (PEM) is an anti-cancer agent targeting DNA and RNA synthesis, and clinically in use for mesothelioma and non-small cell lung carcinoma. A mechanism of resistance to PEM is associated with elevated activities of several enzymes involved in nucleic acid metabolism. Methods We established two kinds of PEM-resistant mesothelioma cells which did not show any increase of the relevant enzyme activities. We screened genes enhanced in the PEM-resistant cells with a microarray analysis and confirmed the expression levels with Western blot analysis. A possible involvement of the candidates in the PEM-resistance was examined with a WST assay after knocking down the expression with si-RNA. We also analyzed a mechanism of the up-regulated expression with agents influencing AMP-activated protein kinase (AMPK) and p53. Results We found that expression of cardiac ankyrin repeat protein (CARP) was elevated in the PEM-resistant cells with a microarray and Western blot analysis. Down-regulation of CARP expression with si-RNA did not however influence the PEM resistance. Parent and PEM-resistant cells treated with PEM increased expression of CARP, AMPK, p53 and histone H2AX. The CARP up-regulation was however irrelevant to the p53 genotypes and not induced by an AMPK activator. Augmented p53 levels with nutlin-3a, an inhibitor for p53 degradation, and DNA damages were not always associated with the enhanced CARP expression. Conclusions These data collectively suggest that up-regulated CARP expression is a potential marker for development of PEM-resistance in mesothelioma and that the PEM-mediated enhanced expression is not directly linked with immediate cellular responses to PEM. |
|||||
書誌情報 |
Cancer cell international 巻 17, p. 120, 発行日 2017-12 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1475-2867 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12050420 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 29238267 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/s12935-017-0493-8 | |||||
権利 | ||||||
権利情報 | © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/ publicdomain/zero/1.0/ ) applies to the data made available in this article, unless otherwise stated. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | BioMed Central |