{"created":"2021-03-01T07:24:19.559992+00:00","id":44607,"links":{},"metadata":{"_buckets":{"deposit":"648cc902-31b6-4f30-8a00-c8d1e47b657e"},"_deposit":{"id":"44607","owners":[],"pid":{"revision_id":0,"type":"depid","value":"44607"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00044607","sets":["2780:1782","2780:918","3:62:5595:5975"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"182","bibliographicPageStart":"178","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Medical journal of Indonesia "}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"加藤, 光保"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"渡邊, 幸秀"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: \nTriple negative breast cancer (TNBC) tends to grow more rapidly and has poorer prognosis compared to others. High expression of transmembrane prostate androgen-induced protein (TMEPAI) correlates with poor prognosis in TNBC patients. However, the mechanistic role of TMEPAI in tumorigenic remains unknown. This study aimed to knock-out TMEPAI in TNBC cell line to determine its function further in cells proliferation.\n\nMethods: \nCRISPR-Cas9 has been used previously to knock-out TMEPAI in Hs857T TNBC cell line. Hs587T TNBC parental cell line (wild-type/WT) and TMEPAI knock out Hs 586T cell lines were cultured in Dulbecco’s modified eagle medium (DMEM) supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin and amphotericin B. Both cell lines were seeded in 24-well plates and counted every two days, then proliferation rates were plotted. Afterwards, total RNA were isolated from the cells and Ki-67, and TGF-β mRNA expression levels as proliferation markers were determined.\n\nResults: \nCell proliferation rates as displayed in growth curve plots showed that WT-TMEPAI cell line grew more rapidly than KO-TMEPAI. In accordance, mRNA expression levels of  Ki-67 and TGF-β  were significantly decreased KO-TMEPAI as compare to TMEPAI-WT.\n\nConclusion: \nKnock-out of TMEPAI attenuates cell proliferation in TNBC.\n","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"University of Indonesia, Faculty of Medicine "}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.13181/mji.v26i3.1823","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"@ 2017 Authors"},{"subitem_rights":"This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are properly cited."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2252-8083","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1113872X ","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wardhani, Bantari W.K."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Puteri, Meidi U."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Yukihide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Louisa, Melva"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Setiabudy, Rianto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Mitsuyasu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-04"}],"displaytype":"detail","filename":"MJI_26-3.pdf","filesize":[{"value":"621.1 kB"}],"format":"application/pdf","licensetype":"license_9","mimetype":"application/pdf","url":{"label":"MJI_26-3","url":"https://tsukuba.repo.nii.ac.jp/record/44607/files/MJI_26-3.pdf"},"version_id":"4e4c17e4-1b54-407f-9486-c70e71df0812"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Knock-out transmembrane prostate androgen-induced protein gene suppressed triple-negative breast cancer cell proliferation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Knock-out transmembrane prostate androgen-induced protein gene suppressed triple-negative breast cancer cell proliferation"}]},"item_type_id":"5","owner":"1","path":["918","1782","5975"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-01-04"},"publish_date":"2018-01-04","publish_status":"0","recid":"44607","relation_version_is_last":true,"title":["Knock-out transmembrane prostate androgen-induced protein gene suppressed triple-negative breast cancer cell proliferation"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:16:03.901601+00:00"}