{"created":"2021-03-01T07:23:57.737323+00:00","id":44270,"links":{},"metadata":{"_buckets":{"deposit":"477b3a23-bbf3-4a80-9299-2474fcdfbf64"},"_deposit":{"id":"44270","owners":[],"pid":{"revision_id":0,"type":"depid","value":"44270"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00044270","sets":["2780:1292","3:62:5592:6181"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-11","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"30","bibliographicPageStart":"25","bibliographicVolumeNumber":"249","bibliographic_titles":[{"bibliographic_title":"Journal of virological methods"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"竹内, 薫"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Two defective bovine parainfluenza virus type 3 (BPIV3) strains were generated, one lacking the membrane (M) protein gene and expressing EGFP (ΔM-EGFP) and the other lacking the fusion (F) protein gene and expressing mStrawberry (ΔF-mSB), by supplying deficient proteins in trans. When Madin-Darby bovine kidney (MDBK) cells were co-infected with ΔM-EGFP and ΔF-mSB at a multiplicity of infection (MOI) of 0.1, complemented viruses were easily obtained. Complemented viruses grew as efficiently as wild-type BPIV3 and could be passaged in MDBK cell cultures even at an MOI of 0.01, possibly due to multiploid virus particles containing genomes of both ΔM-EGFP and ΔF-mSB. This reciprocal complementation method using two defective viruses would be useful to express large or multiple proteins in cell cultures using paramyxovirus vectors.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"28842134","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jviromet.2017.08.010","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ "}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0166-0934","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00259584","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takada, Marina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuura, Ryosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kokuho, Takehiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuboi, Takamitsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kameyama, Ken-ichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takeuchi, Kaoru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-12-01"}],"displaytype":"detail","filename":"JVM_249-25-30.pdf","filesize":[{"value":"466.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"JVM_249-25-30","url":"https://tsukuba.repo.nii.ac.jp/record/44270/files/JVM_249-25-30.pdf"},"version_id":"afd393b4-bcb5-426f-a5a3-fb1d98b96181"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Reciprocal complementation of bovine parainfluenza virus type 3 lacking either the membrane or fusion gene","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Reciprocal complementation of bovine parainfluenza virus type 3 lacking either the membrane or fusion gene"}]},"item_type_id":"5","owner":"1","path":["1292","6181"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-11-27"},"publish_date":"2017-11-27","publish_status":"0","recid":"44270","relation_version_is_last":true,"title":["Reciprocal complementation of bovine parainfluenza virus type 3 lacking either the membrane or fusion gene"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2023-07-12T06:01:37.913272+00:00"}