{"created":"2021-03-01T07:23:04.188285+00:00","id":43460,"links":{},"metadata":{"_buckets":{"deposit":"a6416197-05a2-4cb4-9b40-568ada55e7a2"},"_deposit":{"id":"43460","owners":[],"pid":{"revision_id":0,"type":"depid","value":"43460"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00043460","sets":["160:1242","160:846","3:62:5592:1891"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"102","bibliographicPageStart":"93","bibliographicVolumeNumber":"162","bibliographic_titles":[{"bibliographic_title":"The journal of biochemistry"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中野, 賢太郎"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"沼田, 治"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A contractile ring (CR) is involved in cytokinesis in animal and yeast cells. Although several types of actin-bundling proteins associate with F-actin in the CR, their individual roles in the CR have not yet been elucidated in detail. Ain1 is the sole α-actinin homologue in the fission yeast Schizosaccharomyces pombe and specifically localizes to the CR with a high turnover rate. S. pombe cells lacking the ain1+ gene show defects in cytokinesis under stress conditions. We herein investigated the biochemical activity and cellular localization mechanisms of Ain1. Ain1 showed weaker affinity to F-actin in vitro than other actin-bundling proteins in S. pombe. We identified a mutation that presumably loosened the interaction between two calponin-homology domains constituting the single actin-binding domain (ABD) of Ain1, which strengthened the actin-binding activity of Ain1. This mutant protein induced a deformation in the ring shape of the CR. Neither a truncated protein consisting only of an N-terminal ABD nor a truncated protein lacking a C-terminal region containing an EF-hand motif localized to the CR, whereas the latter was involved in the bundling of F-actin in vitro. We herein propose detailed mechanisms for how each part of the molecule is involved in the proper cellular localization and function of Ain1.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jb/mvx008","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Authors 2017.Published by Oxford University Press on behalf of the Japanese Biochemical Society."},{"subitem_rights":"This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Biochemistry following peer review. The version of record  J Biochem (2017) 162 (2): 93–102  is available online at: https://doi.org/10.1093/jb/mvx008."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-924X","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Morita, Rikuri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takaine, Masak"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Numata, Osamu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakano, Kentaro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-09-01"}],"displaytype":"detail","filename":"JOB_162-2.pdf","filesize":[{"value":"5.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JOB_162-2","url":"https://tsukuba.repo.nii.ac.jp/record/43460/files/JOB_162-2.pdf"},"version_id":"09395c7b-0d41-40ea-a32e-84e56b64630e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Molecular dissection of the actin-binding ability of the fission yeast α-actinin, Ain1, in vitro and in vivo","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Molecular dissection of the actin-binding ability of the fission yeast α-actinin, Ain1, in vitro and in vivo"}]},"item_type_id":"5","owner":"1","path":["1242","846","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-09-27"},"publish_date":"2017-09-27","publish_status":"0","recid":"43460","relation_version_is_last":true,"title":["Molecular dissection of the actin-binding ability of the fission yeast α-actinin, Ain1, in vitro and in vivo"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:14:34.167803+00:00"}