{"created":"2021-03-01T07:23:00.523818+00:00","id":43405,"links":{},"metadata":{"_buckets":{"deposit":"ab9d7e77-cd25-48f7-af25-452030b99dd7"},"_deposit":{"id":"43405","owners":[],"pid":{"revision_id":0,"type":"depid","value":"43405"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00043405","sets":["160:5075","3:62:5296:6094"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"163","bibliographicVolumeNumber":"7","bibliographic_titles":[{},{"bibliographic_title":"AMB Express","bibliographic_titleLang":"en"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"青柳, 秀紀"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Monitoring the environmental factors during shake-flask culture of microorganisms can help to optimise the initial\nsteps of bioprocess development. Herein, we developed a circulation direct monitoring and sampling system\n(CDMSS) that can monitor the behaviour of CO2\nand O2\nin the gas–liquid phases and obtain a sample without interrupting\nthe shaking of the culture in Erlenmeyer flasks capped with breathable culture plugs. Shake-flask culturing of\nEscherichia coli using this set-up indicated that a high concentration of CO2\naccumulated not only in the headspace\n(maximum ~100 mg/L) but also in the culture broth (maximum ~85 mg/L) during the logarithmic phase (4.5–9.0 h).\nBy packing a CO2\nabsorbent in the gas circulation unit of CDMSS, a specialised shake-flask culture was developed to\nremove CO2\nfrom the headspace. It was posited that removing CO2\nfrom the headspace would suppress increases\nin the dissolved CO2\nconcentration in the culture broth (maximum ~15 mg/L). Furthermore, the logarithmic growth\nphase (4.5–12.0 h) was extended, the U.O.D.580 and pH value increased, and acetic acid concentration was reduced,\ncompared with the control. To our knowledge, this is the first report of a method aimed at improving the growth of E.\ncoli cells without changing the composition of the medium, temperature, and shaking conditions.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd "}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"28831757","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s13568-017-0464-4","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2191-0855","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Masato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sawada, Yoshisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aoyagi, Hideki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-21"}],"displaytype":"detail","filename":"AMB_7.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"AMB_7","url":"https://tsukuba.repo.nii.ac.jp/record/43405/files/AMB_7.pdf"},"version_id":"23fb8c67-39aa-4fc3-9d93-cc8478460aa5"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of a circulation direct sampling and monitoring system for O2 and CO2 concentrations in the gas–liquid phases of shake-flask systems during microbial cell culture","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of a circulation direct sampling and monitoring system for O2 and CO2 concentrations in the gas–liquid phases of shake-flask systems during microbial cell culture"}]},"item_type_id":"5","owner":"1","path":["5075","6094"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-09-21"},"publish_date":"2017-09-21","publish_status":"0","recid":"43405","relation_version_is_last":true,"title":["Development of a circulation direct sampling and monitoring system for O2 and CO2 concentrations in the gas–liquid phases of shake-flask systems during microbial cell culture"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:14:29.213673+00:00"}