{"created":"2021-03-01T07:21:20.590989+00:00","id":41854,"links":{},"metadata":{"_buckets":{"deposit":"0fe122df-4f56-4491-b5b0-af52413d27a3"},"_deposit":{"id":"41854","owners":[],"pid":{"revision_id":0,"type":"depid","value":"41854"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00041854","sets":["160:2166","3:62:5587:395"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"815","bibliographicPageStart":"805","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"Extremophiles"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑原 , 朋彦"}],"nameIdentifiers":[{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Thermotogales are rod-shaped, Gram-negative, anaerobic, (hyper) thermophiles distinguished by an outer sheath-like toga, which comprises an outer membrane (OM) and an amorphous layer (AL). Thermosipho globiformans bacteria can transform into spheroids with multiple cells concurrently with AL disintegration during early growth; the cell is defined as the cytoplasmic membrane (CM) plus the entity surrounded by the CM. Spheroids eventually produce rapidly moving periplasmic 'progenies' through an unknown mechanism. Here, we used high-temperature microscopy (HTM) to directly observe spheroid generation and growth. Rod OMs abruptly inflated to form ~2 μm-diameter balloons. Concurrently, multiple globular cells emerged in the balloons, suggesting their translocation and transformation from the rod state. During spheroid growth, the cells elongated and acquired a large dish shape by possible fusion. Spheroids with dish-shaped cells further enlarged to ~12 μm in diameter. HTM and epifluorescence-microscopy results collectively indicated that the nucleoids of dish-shaped cells transformed to form a ring shape, which then distorted to form a lip shape as the spheroid enlarged. HTM showed that 'progenies' were produced in the spheroid periplasm. Transmission electron microscopy results suggested that the 'progenies' represented immature progenies lacking togas, which were acquired subsequently.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer "}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"28577249","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00792-017-0944-0","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2017. This article is an open access publication"},{"subitem_rights":"This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons. org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1431-0651","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11156867 ","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kuwabara, Tomohiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igarashi, Kensuke"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-07-14"}],"displaytype":"detail","filename":"Extremophiles_21-4.pdf","filesize":[{"value":"3.1 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"Extremophiles_21-4","url":"https://tsukuba.repo.nii.ac.jp/record/41854/files/Extremophiles_21-4.pdf"},"version_id":"32c4eb13-fb1a-4e30-b87b-35fddcc320bc"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Progeny production in the periplasm of Thermosipho globiformans","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Progeny production in the periplasm of Thermosipho globiformans"}]},"item_type_id":"5","owner":"1","path":["2166","395"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-07-14"},"publish_date":"2017-07-14","publish_status":"0","recid":"41854","relation_version_is_last":true,"title":["Progeny production in the periplasm of Thermosipho globiformans"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:11:05.546689+00:00"}